A stepwise haematological screening and whole-exome sequencing reveal multiple mutations from SUPT5H causing an elevation of Hb A2 from a cohort of 47336 individuals.
Using mass spectrometry of both MYC and Pol II complexes we show here that MYC controls the assembly of Pol II with a small set of transcription elongation factors that includes SPT5 a subunit of the elongation factor DSIF. MYC directly binds SPT5 recruits SPT5 to promoters and enables the CDK7-dependent transfer of SPT5 onto Pol II
cryo-electron microscopy structure of a paused transcription elongation complex containing Sus scrofa Pol II and Homo sapiens DSIF and NELF at 3.2 A resolution
cryo-EM structure of an activated elongation complex of Sus scrofa Pol II and Homo sapiens DSIF PAF and SPT6 was determined at 3.1 A resolution and compared to the structure of the paused elongation complex formed by Pol II DSIF and NELF
Here the authors identified Legionella pneumophila Lpw27461 as a new nuclear-localised effector that we have termed SnpL. Protein interaction studies showed that SnpL bound to the central Kyprides Ouzounis Woese motif region of SUPT5H. Ectopic expression of SnpL led to massive upregulation of host gene expression and macrophage cell death.
OGA is physically associated with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1beta and a purified OGA-SPT5-TIF1beta complex has elongation properties.
These findings are consistent with a central role of Spt5 in maintenance of TFIID-promoter association and promoter escape to support rapid transcriptional induction and re-initiation of inflammatory-response genes.
Data indicate that the Plus3 domain of the Rtf1 subunit mediates Paf1C recruitment to genes by binding a repeating domain within the phosphorylated elongation factor Spt5.
These results suggest that one of the functions of Spt5 is to suppress senescence and apoptosis and that this function is exerted through its association with Spt4 and Pol II.
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