L1JYR6 · L1JYR6_GUITC
- ProteinRNA-splicing ligase RtcB homolog
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids497 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Miscellaneous
Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.
Catalytic activity
- a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H+
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 124 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 127 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 127 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 231-235 | GMP (UniProtKB | ChEBI) | |||
Binding site | 232 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 264 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 358 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 358-359 | GMP (UniProtKB | ChEBI) | |||
Active site | 420 | GMP-histidine intermediate | |||
Binding site | 420-423 | GMP (UniProtKB | ChEBI) | |||
Binding site | 496 | GMP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | tRNA-splicing ligase complex | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA ligase (ATP) activity | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | tRNA splicing, via endonucleolytic cleavage and ligation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA-splicing ligase RtcB homolog
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Cryptophyceae > Pyrenomonadales > Geminigeraceae > Guillardia
Accessions
- Primary accessionL1JYR6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Proteomic databases
Interaction
Subunit
Catalytic component of the tRNA-splicing ligase complex.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length497
- Mass (Da)54,382
- Last updated2013-03-06 v1
- ChecksumCE135715CEDA8E80
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH992970 EMBL· GenBank· DDBJ | EKX53228.1 EMBL· GenBank· DDBJ | Genomic DNA |