L1JYR6 · L1JYR6_GUITC

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site124Mn2+ 1 (UniProtKB | ChEBI)
Binding site127Mn2+ 1 (UniProtKB | ChEBI)
Binding site127Mn2+ 2 (UniProtKB | ChEBI)
Binding site231-235GMP (UniProtKB | ChEBI)
Binding site232Mn2+ 1 (UniProtKB | ChEBI)
Binding site264Mn2+ 2 (UniProtKB | ChEBI)
Binding site358Mn2+ 2 (UniProtKB | ChEBI)
Binding site358-359GMP (UniProtKB | ChEBI)
Active site420GMP-histidine intermediate
Binding site420-423GMP (UniProtKB | ChEBI)
Binding site496GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Gene names

    • ORF names
      GUITHDRAFT_156993

Organism names

Accessions

  • Primary accession
    L1JYR6

Proteomes

Subcellular Location

PTM/Processing

Proteomic databases

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    497
  • Mass (Da)
    54,382
  • Last updated
    2013-03-06 v1
  • Checksum
    CE135715CEDA8E80
MVVRTYEEECKYVKQVDGTCYRIDKGFVDNMRVPGVFYVNQQIKELLFDELKQFSKTSGAAAGMGGFLPAMKQLANVAALPGIVGKSIALPDVHAGYGFAIGNVAAFDMSDPEAVVSPGGVGFDINCGVRLIRTNLFEKDVEPVKEKLAQAVFDHIPVGVGSQGIIPTNSNDLEEALEMGIDWSLREGYSWPEDKEHCEEYGRMLQADPRKVSSRAKKRGLPQLGTLGAGNHYAEIQVVDEIFDARAASQMGIEEKGQVVVMIHSGSRGLGHQVATDALTAMDKAMDRDNIRTNDRQLSCARIDSQEGQDYLAAMAAAANYAWVNRSSMTFLVRQAFAKVFQSTPDDLDMHLIYDVSHNIAKVEEHMVDGNIKKLLVHRKGSTRAFPPHHPLIPVDYQFTGQPVLIGPAGMEETFGSTCHGAGRALSRNKSRNNLDYKDVLQVLQDKGIAIRVASPKLVMEEAPESYKDVTQVVDTCQAAGISNKVVKLRPIAVIKG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JH992970
EMBL· GenBank· DDBJ
EKX53228.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help