L0TH62 · L0TH62_TECGR
- ProteinDNA-directed RNA polymerase subunit beta''
- GenerpoC
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1395 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 224 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 295 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 302 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 305 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta''
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Lamiaceae > Tectonoideae > Tectona
Accessions
- Primary accessionL0TH62
Subcellular Location
Interaction
Subunit
In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 97-161 | RNA polymerase Rpb1 | |||
Domain | 176-377 | RNA polymerase Rpb1 | |||
Region | 501-523 | Disordered | |||
Compositional bias | 508-523 | Polar residues | |||
Domain | 1198-1283 | RNA polymerase Rpb1 | |||
Sequence similarities
Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,395
- Mass (Da)158,119
- Last updated2013-03-06 v1
- Checksum90C89B50726C8E1B
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 508-523 | Polar residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HF567869 EMBL· GenBank· DDBJ | CCP47120.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HF567870 EMBL· GenBank· DDBJ | CCP47209.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HF567871 EMBL· GenBank· DDBJ | CCP47298.1 EMBL· GenBank· DDBJ | Genomic DNA |