K8Y140 · K8Y140_9LEPT
- ProteinAdenosine 5'-phosphosulfate reductase
- GenecysH
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids245 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.
Catalytic activity
- [thioredoxin]-disulfide + sulfite + AMP + 2 H+ = adenosine 5'-phosphosulfate + [thioredoxin]-dithiol
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:17359 + CHEBI:456215 + 2 CHEBI:15378 = CHEBI:58243 + RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3
Cofactor
Note: Binds 1 [4Fe-4S] cluster per subunit.
Pathway
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 115 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 116 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 198 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 201 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Active site | 225 | Nucleophile; cysteine thiosulfonate intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoadenylyl-sulfate reductase (thioredoxin) activity | |
Biological Process | sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenosine 5'-phosphosulfate reductase
- EC number
- Short namesAPS reductase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Spirochaetota > Spirochaetia > Leptospirales > Leptospiraceae > Leptospira
Accessions
- Primary accessionK8Y140
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length245
- Mass (Da)28,084
- Last updated2013-02-06 v1
- Checksum45BB381F7CD51997
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP006694 EMBL· GenBank· DDBJ | EKT87353.1 EMBL· GenBank· DDBJ | Genomic DNA |