K7XK97 · K7XK97_9ALPC

  • Protein
    ORF1a polyprotein
  • Gene
    1a
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
    EC:3.4.19.12 (UniProtKB | ENZYME | Rhea)

GO annotations

AspectTerm
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Submitted names
    • ORF1a polyprotein

Gene names

    • Name
      1a

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • A76
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Tegacovirus > Alphacoronavirus 1

Accessions

  • Primary accession
    K7XK97

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1834-1852Helical
Transmembrane1889-1909Helical
Transmembrane1971-1988Helical
Transmembrane1995-2013Helical
Transmembrane2025-2045Helical
Transmembrane2400-2420Helical
Transmembrane2665-2685Helical
Transmembrane2690-2707Helical
Transmembrane2713-2731Helical
Transmembrane2743-2767Helical
Transmembrane3186-3205Helical
Transmembrane3217-3236Helical
Transmembrane3242-3266Helical
Transmembrane3287-3306Helical
Transmembrane3312-3330Helical
Transmembrane3370-3389Helical
Transmembrane3396-3414Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond1920↔1947
Disulfide bond1938↔1944

Keywords

Interaction

Subunit

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer.

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain2-108CoV Nsp1 globular
Domain111-349CoV Nsp2 N-terminal
Domain378-773CoV Nsp2 middle
Domain768-879CoV Nsp2 C-terminal
Domain882-983Ubiquitin-like
Compositional bias1002-1021Acidic residues
Region1002-1039Disordered
Domain1054-1298Peptidase C16
Region1284-1314Disordered
Domain1317-1488Macro
Domain1485-1541Ubiquitin-like
Domain1549-1802Peptidase C16
Domain1904-19693Ecto
Region2043-2133Y1
Domain2043-2383CoV Nsp3 Y
Region2047-2060ZF1
Region2093-2103ZF2
Region2134-2383CoV-Y
Region2281-2383Y4
Domain2782-2877Nsp4C
Domain2878-3179Peptidase C30
Domain3474-3556RdRp Nsp7 cofactor
Domain3557-3751RdRp Nsp8 cofactor
Domain3752-3862Nsp9 ssRNA-binding
Domain3863-4003ExoN/MTase coactivator

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    4,016
  • Mass (Da)
    447,798
  • Last updated
    2013-02-06 v1
  • Checksum
    D2BE2BF3B2F25503

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1002-1021Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JN856008
EMBL· GenBank· DDBJ
AFX65759.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp