K7SLC7 · K7SLC7_ACIA4

Function

function

Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 3 Mg2+ ions per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site990Mg2+ 1 (UniProtKB | ChEBI)
Binding site997Mg2+ 2 (UniProtKB | ChEBI)
Binding site997Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionlysine-tRNA ligase activity
Molecular Functionmagnesium ion binding
Molecular Functionphosphatidylglycerol lysyltransferase activity
Molecular FunctiontRNA binding
Biological Processlipid metabolic process
Biological Processlysyl-tRNA aminoacylation
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lysine--tRNA ligase
  • EC number
  • Alternative names
    • Lysyl-tRNA synthetase
      (LysRS
      )

Gene names

    • Name
      lysS
    • Ordered locus names
      PACID_23030

Organism names

Accessions

  • Primary accession
    K7SLC7

Proteomes

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane25-48Helical
Transmembrane60-82Helical
Transmembrane102-123Helical
Transmembrane135-156Helical

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain755-1077Aminoacyl-transfer RNA synthetases class-II family profile

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the LPG synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,081
  • Mass (Da)
    117,723
  • Last updated
    2013-02-06 v1
  • Checksum
    E624299926B269E6
MLVGAVLLIGRMLFQQTWWVSALATGFSIVVFPIDGGGWGLVVLLLILAAGLNRRKRLSWAVCLGLFGLMWVVSLLFFGVLIAEVAGGEPTRIHDDVEMAGYVFNTFSVGAILVALIVHHGRFGARTARANVRPALATLVGGLAATVLIGWLLVWATGGQGRPRNRLWRLISELLRGNRSQVAPPSPIWVENIVELLAAITLATAFIMLLRSQRQVAVLGLGDELELRNLLDENPSDSLGYFALRRDKAVVFSPDRRAAVCYRAVAGVALASGDPVGPRAQWPAAIDAFLTVAHTYGWSPAVVGASEEGATAWQGAGLRVTRIGDEAIISPSTFDLQSRDLRGVRSVVNRLRRSGYTVRVRRHSDIEPDELSRLIALADAWRADGEERGFSMALSRLGDPLDGDCVMVEALYPAGDIRGDGSTAGLLSFVPWGGDGLSLDVMRHDPRADNGVTELMVAGLMATGRELGLHRVSLNFAVFREVIEEGSRVGARPLQRLAARFVSGVSRWFQIEQLYRSNVKYAPDWQARYLGYADAGELAQVGLALGLAEGQIDLPRILRPAPPPAQPIYSVAAHPEIAAVLERQRPDVELPGRRVGEQMRHRQQTRERIIAAGGEAYPPDIHPSGAPGDLATAAEGGELSLVGRVAGVRDHGGVIFADLTDWSGTAQILLDASALGAPAMDRWRADISLGDHVLVGGRAGSSRNGTRSLLAGEFRLAAKALHPLPDRRSGMSDPEEKVRRRYLDLIVNPAARDELRARSQAIRAVRETLLSHDFLEVETPILQTIHGGANARPFRTHINAYDLDLYLRIAPELYLKRLMVGGAGRVFEIGRNFRNEGADATHNPEFTMLEAYQAYGDYVQMRHLTREMILAASRRATGGTVIHGRDADGVEHEMDLAEEWRVITVNGGISAALGEEVTADTPKETLVGYAEKLGIAVSPKWDRGDVVLELHEHLSERTTVGPTFFCDFPADVSPLTRQHRDDPRLAEKWDLIVLGDEVATAYTELVDPVIQRERFTAQSLRAAGGDPEAMELDEDFLEALEYAMPPTGGMGMGLDRLVMLLTGASIRQTITFPLVRPRSSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003493
EMBL· GenBank· DDBJ
AFV90085.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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