K4CJJ1 · NCED2_SOLLC
- Protein9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
- GeneNCED2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids581 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid (ABA) biosynthesis from carotenoids.
Catalytic activity
- a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Pathway
Plant hormone biosynthesis; abscisate biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | 9-cis-epoxycarotenoid dioxygenase activity | |
Molecular Function | carotenoid dioxygenase activity | |
Molecular Function | metal ion binding | |
Biological Process | abscisic acid biosynthetic process | |
Biological Process | carotene catabolic process | |
Biological Process | response to abscisic acid | |
Biological Process | response to water deprivation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
- EC number
- Short namesLeNCED2 ; SlNCED2
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionK4CJJ1
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Partially bound to the thylakoid.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-82 | Chloroplast | ||||
Sequence: MTSTIANYRVSHSFSPSTSYSLDFTLPSKSISMKNHTTTKTKIHSALLTLPKQNNTPKNQPQFQTSHWNFFQKAAAKALDIV | ||||||
Chain | PRO_0000445688 | 83-581 | 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic | |||
Sequence: ESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQV |
Proteomic databases
Expression
Tissue specificity
Expressed in flowers (PubMed:29632966).
Expressed in developing and ripening fruits (PubMed:22108525, PubMed:22345638).
Expressed in developing and ripening fruits (PubMed:22108525, PubMed:22345638).
Induction
Induced by abscisic-acid (ABA) and dehydration.
Developmental stage
Expressed in the meristem during gametogenesis and mainly in ovule, stigma, anther/pollen and vascular tissues during flower development. In flower buds, present in petal primordium and apical meristem. Later expressed in stamen primordium and pistil meristem after petal formation. Accumulates in stamen, pistil and vascular tissues of the bud base. Highly expressed in sporogenous cells in anther, pistil stigma, ovules, pollen grains, anther walls, and basal vascular bundle. In anthers, first present at high levels, then declines rapidly from stage 13 (when microspores transform into vacuolated pollen grains after mitotic divisions) to stage 16 (when the sepals are better separated, and the faint yellow petals are clearer) to a very low level (PubMed:29632966).
In fruits, expressed at high levels at the immature stage and then declined continually through all stages of fruit development (PubMed:22108525, PubMed:22345638, PubMed:25039074).
In fruits, expressed at high levels at the immature stage and then declined continually through all stages of fruit development (PubMed:22108525, PubMed:22345638, PubMed:25039074).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length581
- Mass (Da)64,834
- Last updated2012-11-28 v1
- Checksum8E9F510AC75D666C
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 335 | in Ref. 2; ACL00683 | ||||
Sequence: N → S | ||||||
Sequence conflict | 449 | in Ref. 2; ACL00683 | ||||
Sequence: L → P |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001071 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
EU912387 EMBL· GenBank· DDBJ | ACL00683.2 EMBL· GenBank· DDBJ | mRNA |