K4CJJ1 · NCED2_SOLLC

Function

function

Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid (ABA) biosynthesis from carotenoids.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Pathway

Plant hormone biosynthesis; abscisate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site279Fe cation (UniProtKB | ChEBI)
Binding site328Fe cation (UniProtKB | ChEBI)
Binding site393Fe cation (UniProtKB | ChEBI)
Binding site568Fe cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast stroma
Molecular Function9-cis-epoxycarotenoid dioxygenase activity
Molecular Functioncarotenoid dioxygenase activity
Molecular Functionmetal ion binding
Biological Processabscisic acid biosynthetic process
Biological Processcarotene catabolic process
Biological Processresponse to abscisic acid
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic
  • EC number
  • Short names
    LeNCED2
    ; SlNCED2
  • Alternative names
    • Nine-cis-epoxycarotenoid dioxygenase 2

Gene names

    • Name
      NCED2
    • Ordered locus names
      Solyc08g016720.1.1

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Heinz 1706
    • cv. Ailsa Craig
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon

Accessions

  • Primary accession
    K4CJJ1
  • Secondary accessions
    • C5H805

Proteomes

Genome annotation databases

Subcellular Location

Note: Partially bound to the thylakoid.

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-82Chloroplast
ChainPRO_000044568883-5819-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic

Proteomic databases

Expression

Tissue specificity

Expressed in flowers (PubMed:29632966).
Expressed in developing and ripening fruits (PubMed:22108525, PubMed:22345638).

Induction

Induced by abscisic-acid (ABA) and dehydration.

Developmental stage

Expressed in the meristem during gametogenesis and mainly in ovule, stigma, anther/pollen and vascular tissues during flower development. In flower buds, present in petal primordium and apical meristem. Later expressed in stamen primordium and pistil meristem after petal formation. Accumulates in stamen, pistil and vascular tissues of the bud base. Highly expressed in sporogenous cells in anther, pistil stigma, ovules, pollen grains, anther walls, and basal vascular bundle. In anthers, first present at high levels, then declines rapidly from stage 13 (when microspores transform into vacuolated pollen grains after mitotic divisions) to stage 16 (when the sepals are better separated, and the faint yellow petals are clearer) to a very low level (PubMed:29632966).
In fruits, expressed at high levels at the immature stage and then declined continually through all stages of fruit development (PubMed:22108525, PubMed:22345638, PubMed:25039074).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the carotenoid oxygenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    581
  • Mass (Da)
    64,834
  • Last updated
    2012-11-28 v1
  • Checksum
    8E9F510AC75D666C
MTSTIANYRVSHSFSPSTSYSLDFTLPSKSISMKNHTTTKTKIHSALLTLPKQNNTPKNQPQFQTSHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict335in Ref. 2; ACL00683
Sequence conflict449in Ref. 2; ACL00683

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM001071
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
EU912387
EMBL· GenBank· DDBJ
ACL00683.2
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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