K2RU68 · DPMPH_MACPH
- ProteinShort chain dehydrogenase/reductase dpmpH
- GenedpmpH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids203 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of diterpenoid pyrones (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpmpA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpmpD through the action of the prenyltransferase dpmpC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpmpE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpmpB (Probable). The short chain dehydrogenase/reductase dpmpG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpmpH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Higginsianin B is further methylated by the methyltransferase dpmpI to produce the intermediate named FDDP B (PubMed:32286350).
The cytochrome P450 monooxygenase dpmpJ then oxidizes the C-26 methyl to primary alcohol, producing the final diterpenoid pyrone with a C-26 primary alcohol on the gamma-pyrone moiety named FDDP C (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpmpA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpmpD through the action of the prenyltransferase dpmpC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpmpE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpmpB (Probable). The short chain dehydrogenase/reductase dpmpG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpmpH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Higginsianin B is further methylated by the methyltransferase dpmpI to produce the intermediate named FDDP B (PubMed:32286350).
The cytochrome P450 monooxygenase dpmpJ then oxidizes the C-26 methyl to primary alcohol, producing the final diterpenoid pyrone with a C-26 primary alcohol on the gamma-pyrone moiety named FDDP C (PubMed:32286350).
Biotechnology
Diterpenoid pyrones display various biological activities and FDDP C shows anti-cancer and anti-HIV activities (PubMed:32286350).
FDDP C shows also inhibitory activity of 42-mer-amyloid beta aggregation that is involved in the pathogenesis of Alzheimer's disease (PubMed:32286350).
FDDP C shows also inhibitory activity of 42-mer-amyloid beta aggregation that is involved in the pathogenesis of Alzheimer's disease (PubMed:32286350).
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameShort chain dehydrogenase/reductase dpmpH
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetes incertae sedis > Botryosphaeriales > Botryosphaeriaceae > Macrophomina
Accessions
- Primary accessionK2RU68
Proteomes
Organism-specific databases
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451552 | 1-203 | Short chain dehydrogenase/reductase dpmpH | |||
Sequence: MSAIGHSPTACSRCSRPRLRRKDLTSVLSPRLPCFKVSSSGEAFATDKADFITPKDLDDPCASPGWEASSLAQAKRYGKSKMANVLFAAELQRRMDAEGVDIISISLNPGPVKTQGAADVLPFMVRPMVWLFFKDPAEGAQTTLFAAAAAEIREEKERWKGGYLDGPGKLKSPSPRARDPQVAYNLWHTTESAVRAIGVLDKS |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length203
- Mass (Da)22,081
- Last updated2012-11-28 v1
- Checksum717E3AB7F7BC9A1B
Keywords
- Technical term