K2RU68 · DPMPH_MACPH

Function

function

Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of diterpenoid pyrones (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpmpA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpmpD through the action of the prenyltransferase dpmpC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpmpE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpmpB (Probable). The short chain dehydrogenase/reductase dpmpG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpmpH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Higginsianin B is further methylated by the methyltransferase dpmpI to produce the intermediate named FDDP B (PubMed:32286350).
The cytochrome P450 monooxygenase dpmpJ then oxidizes the C-26 methyl to primary alcohol, producing the final diterpenoid pyrone with a C-26 primary alcohol on the gamma-pyrone moiety named FDDP C (PubMed:32286350).

Biotechnology

Diterpenoid pyrones display various biological activities and FDDP C shows anti-cancer and anti-HIV activities (PubMed:32286350).
FDDP C shows also inhibitory activity of 42-mer-amyloid beta aggregation that is involved in the pathogenesis of Alzheimer's disease (PubMed:32286350).

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site23NADP+ (UniProtKB | ChEBI)
Active site77Proton acceptor
Binding site77NADP+ (UniProtKB | ChEBI)
Active site81Lowers pKa of active site Tyr
Binding site81NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Short chain dehydrogenase/reductase dpmpH
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein H

Gene names

    • Name
      dpmpH
    • ORF names
      MPH_09200

Organism names

Accessions

  • Primary accession
    K2RU68

Proteomes

Organism-specific databases

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004515521-203Short chain dehydrogenase/reductase dpmpH

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    203
  • Mass (Da)
    22,081
  • Last updated
    2012-11-28 v1
  • Checksum
    717E3AB7F7BC9A1B
MSAIGHSPTACSRCSRPRLRRKDLTSVLSPRLPCFKVSSSGEAFATDKADFITPKDLDDPCASPGWEASSLAQAKRYGKSKMANVLFAAELQRRMDAEGVDIISISLNPGPVKTQGAADVLPFMVRPMVWLFFKDPAEGAQTTLFAAAAAEIREEKERWKGGYLDGPGKLKSPSPRARDPQVAYNLWHTTESAVRAIGVLDKS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AHHD01000387
EMBL· GenBank· DDBJ
EKG13734.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp