K0JXB9 · K0JXB9_SACES

Function

function

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

S-adenosyl-L-methionine (UniProtKB | Rhea| CHEBI:59789 )

Note: Binds 1 S-adenosyl-L-methionine per subunit.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site12-14substrate
Binding site27substrate
Binding site31[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site46[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site48-50S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site49[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site51Mg2+ (UniProtKB | ChEBI)
Binding site91substrate
Binding site93S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site134-136S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site174-177S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-nitrogen lyase activity
Molecular Functionmagnesium ion binding
Molecular FunctionS-adenosyl-L-methionine binding
Biological Processqueuosine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    7-carboxy-7-deazaguanine synthase
  • EC number
  • Short names
    CDG synthase
  • Alternative names
    • Queuosine biosynthesis protein QueE

Gene names

    • Name
      queE
    • Ordered locus names
      BN6_20840

Organism names

Accessions

  • Primary accession
    K0JXB9

Proteomes

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-211Radical SAM core

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    211
  • Mass (Da)
    23,441
  • Last updated
    2012-11-28 v1
  • Checksum
    98F7A89080815832
MTYLVKEIFYTLQGEGAHSGRPAVFCRFSRCNLWTGHEKDRARAVCRFCDTDFVGIDGPGGGRFATPAELADAVEAAWPTLGKARRFVVCTGGEPLLQLDEVAVQTLHERGFEVAVETNGTRVPPPGIDWLCVSPKVGAELVVESGDELKFVYPQEGVNPARFEHLDFRSFRLQPLDDHNLDDNTKAAISYCLANPRWSLSLQTHKYLGTR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HE804045
EMBL· GenBank· DDBJ
CCH29404.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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