K0JXB9 · K0JXB9_SACES
- Protein7-carboxy-7-deazaguanine synthase
- GenequeE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids211 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
Catalytic activity
- 6-carboxy-5,6,7,8-tetrahydropterin + H+ = 7-carboxy-7-deazaguanine + NH4+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 S-adenosyl-L-methionine per subunit.
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-14 | substrate | ||||
Sequence: LQG | ||||||
Binding site | 27 | substrate | ||||
Sequence: R | ||||||
Binding site | 31 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 46 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 48-50 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: FCD | ||||||
Binding site | 49 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 51 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 91 | substrate | ||||
Sequence: T | ||||||
Binding site | 93 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 134-136 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: SPK | ||||||
Binding site | 174-177 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: QPLD |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | carbon-nitrogen lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | queuosine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name7-carboxy-7-deazaguanine synthase
- EC number
- Short namesCDG synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Saccharothrix
Accessions
- Primary accessionK0JXB9
Proteomes
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 18-211 | Radical SAM core | ||||
Sequence: HSGRPAVFCRFSRCNLWTGHEKDRARAVCRFCDTDFVGIDGPGGGRFATPAELADAVEAAWPTLGKARRFVVCTGGEPLLQLDEVAVQTLHERGFEVAVETNGTRVPPPGIDWLCVSPKVGAELVVESGDELKFVYPQEGVNPARFEHLDFRSFRLQPLDDHNLDDNTKAAISYCLANPRWSLSLQTHKYLGTR |
Sequence similarities
Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length211
- Mass (Da)23,441
- Last updated2012-11-28 v1
- Checksum98F7A89080815832
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HE804045 EMBL· GenBank· DDBJ | CCH29404.1 EMBL· GenBank· DDBJ | Genomic DNA |