K0IVJ1 · K0IVJ1_9VIRU

  • Protein
    Pre-glycoprotein polyprotein GP complex
  • Gene
    GP
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Glycoprotein G1

Forms the virion spikes together with glycoprotein G2. The glycoprotein spike trimers are connected to the underlying matrix. Interacts with the host receptor leading to virus endocytosis.

Glycoprotein G2

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification.

Stable signal peptide

Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site57Zn2+ 1 (UniProtKB | ChEBI)
Site58-59Cleavage; by host signal peptidase
Site259-260Cleavage; by host MBTPS1
Binding site455Zn2+ 2 (UniProtKB | ChEBI)
Binding site457Zn2+ 2 (UniProtKB | ChEBI)
Binding site463Zn2+ 2 (UniProtKB | ChEBI)
Binding site467Zn2+ 1 (UniProtKB | ChEBI)
Binding site475Zn2+ 1 (UniProtKB | ChEBI)
Binding site477Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell Golgi membrane
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Cellular Componentviral envelope
Cellular Componentvirion membrane
Molecular Functionmetal ion binding
Biological Processfusion of virus membrane with host endosome membrane
Biological Processreceptor-mediated endocytosis of virus by host cell
Biological Processvirion attachment to host cell

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Pre-glycoprotein polyprotein GP complex
  • Short names
    Pre-GP-C
  • Cleaved into 3 chains

Gene names

    • Name
      GP
    • Synonyms
      GPC

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • LSK-3
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Arenaviridae > Mammarenavirus

Accessions

  • Primary accession
    K0IVJ1

Proteomes

Subcellular Location

Glycoprotein G2

Virion membrane
; Single-pass membrane protein
Host endoplasmic reticulum membrane
; Single-pass membrane protein
Host Golgi apparatus membrane
; Single-pass membrane protein
Host cell membrane
; Single-pass membrane protein
Note: Binding to the stable signal peptide masks endogenous ER localization signals in the cytoplasmic domain of G2 to ensure that only the fully assembled, tripartite GP complex is transported for virion assembly.

Glycoprotein G1

Virion membrane
; Peripheral membrane protein
Host endoplasmic reticulum membrane
; Peripheral membrane protein
Host Golgi apparatus membrane
; Peripheral membrane protein
Host cell membrane
; Peripheral membrane protein

Stable signal peptide

Virion membrane
; Single-pass type II membrane protein
Host endoplasmic reticulum membrane
; Single-pass type II membrane protein
Host Golgi apparatus membrane
; Single-pass type II membrane protein
Host cell membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain2-17Extracellular
Transmembrane21-52Helical
Topological domain34-58Cytoplasmic
Topological domain59-432Extracellular
Transmembrane428-449Helical
Topological domain454-491Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for initiator methionine, lipidation, chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
Initiator methionine1Removed; by host
Lipidation2N-myristoyl glycine; by host
ChainPRO_50235211112-58Stable signal peptide
ChainPRO_50235211122-491Pre-glycoprotein polyprotein GP complex
ChainPRO_503652187659-259Glycoprotein G1
Glycosylation78N-linked (GlcNAc...) asparagine; by host
Glycosylation88N-linked (GlcNAc...) asparagine; by host
Glycosylation98N-linked (GlcNAc...) asparagine; by host
Glycosylation108N-linked (GlcNAc...) asparagine; by host
Disulfide bond117↔154
Glycosylation118N-linked (GlcNAc...) asparagine; by host
Glycosylation166N-linked (GlcNAc...) asparagine; by host
Glycosylation224N-linked (GlcNAc...) asparagine; by host
ChainPRO_5023521113260-491Glycoprotein G2
Disulfide bond279↔292
Disulfide bond301↔310
Disulfide bond364↔385
Glycosylation365N-linked (GlcNAc...) asparagine; by host
Glycosylation373N-linked (GlcNAc...) asparagine; by host
Glycosylation390N-linked (GlcNAc...) asparagine; by host
Glycosylation395N-linked (GlcNAc...) asparagine; by host

Post-translational modification

Pre-glycoprotein polyprotein GP complex

Specific enzymatic cleavages in vivo yield mature proteins. GP-C polyprotein is cleaved in the endoplasmic reticulum by the host protease MBTPS1. Only cleaved glycoprotein is incorporated into virions. The SSP remains stably associated with the GP complex following cleavage by signal peptidase.

Keywords

Interaction

Subunit

Glycoprotein G1

Homotrimer; disulfide-linked. In pre-fusion state, G1 homotrimers bind G2 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G2 and the stable signal peptide. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Glycoprotein G2

Homotrimer. Interacts with the stable signal peptide. In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide. Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Stable signal peptide

Interacts with glycoprotein G2. Part of the GP complex (GP-C) together with glycoprotein G1 and glycoprotein G2. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region258-294Fusion
Region295-363HR1
Region368-431HR2

Domain

Glycoprotein G2

Contains 1 fusion peptide at the N-terminus, 2 heptad repeats domains HR1 and HR2 and, at the C-terminus, a cytoplasmic domain that plays a role in ER location. Also contains a zinc-binding domain that allows SSP retention in the GPC complex by accepting a cysteine from SSP as the fourth ligand.

Stable signal peptide

The N-terminus is localized at the extracellular side of the GP-C, with a part embedded in the membrane probably.

Sequence similarities

Belongs to the arenaviridae GPC protein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    491
  • Mass (Da)
    56,179
  • Last updated
    2012-11-28 v1
  • Checksum
    84D51D99896BF803
MGQIITFFQEVPHIVEEVMNIVLITLSLLAILKGIYNLATCGLLGLISFLFLCGRSCTLVYKDNYALNSVELDMSTLNFTMPLSCSKNNTHHYIQTYNDSGLELTLTNMSILNHKFCNLSDAHRKNLYDHTLMSVVTTFHLSIPNFNQYESMACDFDGGKITVQYNLSHSTPVEAANHCGTVANGVLDTFRRMHWGPAHFPNPPFTIGGTSCIQTSYQYLIIQNTTWEDHCQMSRPTPMGYLSLIAQRTRQLYISRRLMGTFTWTLSDSSGNDLPGGYCLQRWMLIEAEMKCFGNTAVAKCNQQHDEEFCDMLRLFDFNKEAIKRLKTEAEKSINLINKAVNSLINDQLIIRNHLRDLMGIPYCNYSKFWYLNDTRNGKTSLPKCWKVSNNSYLNETHFSNEIEEEANNMITEMLRKEYEKRQSTTPLGLVDLFVFSTSFYLITVFLHLMKIPTHRHIVGKGCPKPHRLNHKAVCSCGAFGQPGLPVRWKR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB702940
EMBL· GenBank· DDBJ
BAM42782.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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