K0I210 · FOMT4_OCIBA
- ProteinFlavonoid 6-O-methyltransferase 4
- GeneFOMT4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids336 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Flavonoid 6-O-methyltransferase involved in the biosynthesis of polymethoxylated flavonoids natural products such as nevadensin and salvigenin (SALV), aroma compounds which contribute to the flavor of sweet basil, and exhibit pharmacological activities such as anti-allergic, anti-oxidant, antibacterial, anti-proliferative, and anti-inflammatory effects (PubMed:22923679).
Catalyzes S-adenosylmethionine-dependent regioselective 6-O-methylation of flavonoids; active on various hydroxylated flavonoid substrates, including scutellarein-7-methyl ether (SCU7Me) and ladanein (LAD) (PubMed:22923679).
Catalyzes S-adenosylmethionine-dependent regioselective 6-O-methylation of flavonoids; active on various hydroxylated flavonoid substrates, including scutellarein-7-methyl ether (SCU7Me) and ladanein (LAD) (PubMed:22923679).
Catalytic activity
- ladanein + S-adenosyl-L-methionine = H+ + S-adenosyl-L-homocysteine + salvigeninThis reaction proceeds in the forward direction.
- S-adenosyl-L-methionine + scutellarein 7-methyl ether = cirsimaritin + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Biotechnology
Nevadensin is a selective inhibitor of human carboxylesterase 1 (hCE-1), a key enzyme responsible for the hydrolysis of a wide range of endogenous and xenobiotic esters.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
98 nM | scutellarein-7-methyl ether | in the presence of S-adenosyl-L-methionine | ||||
54 nM | ladanein | in the presence of S-adenosyl-L-methionine | ||||
21 μM | S-adenosyl-L-methionine | in the presence of scutellarein |
kcat is 130x10-3 sec-1 with scutellarein-7-methyl ether as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679).
kcat is 140x10-3 sec-1 with ladanein as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679).
kcat is 140x10-3 sec-1 with ladanein as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:22923679).
Pathway
Flavonoid metabolism.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-methyltransferase activity | |
Molecular Function | protein dimerization activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavonoid 6-O-methyltransferase 4
- EC number
- Short namesObFOMT4
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Lamiaceae > Nepetoideae > Ocimeae > Ociminae > Ocimum
Accessions
- Primary accessionK0I210
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 292 | Slight acquired ability to produce SALV from scutellarein-7-methyl ether (SCU7Me); when associated with V-296; M-297 and A-298. Acquired ability to produce salvigenin (SALV), and slightly ladanein (LAD) from scutellarein-7-methyl ether (SCU7Me); when associated with V-296; M-297 and A-298. | ||||
Sequence: T → I | ||||||
Mutagenesis | 296 | Slight acquired ability to produce SALV from scutellarein-7-methyl ether (SCU7Me); when associated with I-292; M-297 and A-298. Acquired ability to produce salvigenin (SALV), and slightly ladanein (LAD) from scutellarein-7-methyl ether (SCU7Me); when associated with I-292; M-297 and A-298. | ||||
Sequence: M → V | ||||||
Mutagenesis | 297 | Slight acquired ability to produce SALV from scutellarein-7-methyl ether (SCU7Me); when associated with I-292; V-296 and A-298. Acquired ability to produce salvigenin (SALV), and slightly ladanein (LAD) from scutellarein-7-methyl ether (SCU7Me); when associated with I-292; V-296 and A-298. | ||||
Sequence: T → M | ||||||
Mutagenesis | 298 | Acquired ability to produce salvigenin (SALV), and slightly ladanein (LAD) from scutellarein-7-methyl ether (SCU7Me); when associated with I-292; V-296 and M-297. | ||||
Sequence: T → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000456917 | 1-336 | Flavonoid 6-O-methyltransferase 4 | |||
Sequence: MAVDKEVELHAQAWDHALSYITPTALSAAVELEIPDILEDHGGLMSLSELSAASGCPREPLYRLMRFLIFHGIFTKSNDCYAQSPLSRVFTRENLGPYMLMQATPVTRSPAGLSGEALKTGTPLYLKSIRGEDSWNDPAYGFHMRAFTNGMAAHARLTAAAIVTNYPTAFNGVRSVVDVGGRHGMAIGKLVEAFPWVRGIAFDLPEVVADAPPRKGVDFVGGDMFESLPKADAVMLMWVLHDWSDDKCIEILKKCKEAIPTSTGKVMIVDAIINEEGEGDEFSGARLSLDMTMMAMTTQGKERSYKEWVHLLNEAGFSKHTVKNIKTIEFVIEAYP |
Expression
Tissue specificity
Expressed in leaves.
Developmental stage
Accumulates in young leaves but fades out during leaves aging.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length336
- Mass (Da)36,961
- Last updated2012-11-28 v1
- ChecksumE4AAF2EAC993B286