Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

J9SRG0 · J9SRG0_9ACTN

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Features

Showing features for binding site, active site.

111291002003004005006007008009001,0001,100
Type
IDPosition(s)Description
Binding site117ATP (UniProtKB | ChEBI)
Binding site200ATP (UniProtKB | ChEBI)
Binding site235ATP (UniProtKB | ChEBI)
Active site292
Binding site535Mn2+ (UniProtKB | ChEBI)
Binding site607substrate
Binding site705Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site734Mn2+ (UniProtKB | ChEBI)
Binding site736Mn2+ (UniProtKB | ChEBI)
Binding site869substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • ORF names
      KTR9_4331

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KTR9
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia

Accessions

  • Primary accession
    J9SRG0

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue705N6-carboxylysine
Modified residue1095N6-biotinyllysine

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-449Biotin carboxylation
Domain121-317ATP-grasp
Region478-505Disordered
Domain526-795Pyruvate carboxyltransferase

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,129
  • Mass (Da)
    120,337
  • Last updated
    2012-11-28 v1
  • MD5 Checksum
    F9AC5EA5ABAB4441B47543925002BB36
MFDKILVANRGEIAVRAFRAAYELGAATVAVFPYEDRNSTHRLKADESYQIGAPGHPVRAYLSVEEIVAAARRSGSDAIYPGYGFLSENPDLSAACEAAGLTFIGPPTEVLELTGNKARAVAAAREAGLPVLASSAPSADVDELLAAAEAMSFPVFVKAVAGGGGRGMRRVAEAEELGEAIRAASREAESAFGDPTVFLEQAVINPRHIEVQILGDTQGNVIHLFERDCSLQRRHQKVIELAPAPNLSAELRAKICADAVAFARHIGYSCAGTVEFLLDERGQHVFIEMNPRIQVEHTVTEEITDVDLVQSQMRIASGETLADLGLSQDRLQIRGAALQCRITTEDPTNGFRPDTGRITAYRTPGGAGVRLDGGATLGAEVSAHFDSMLVKLTCRGRDFDTAVARARRAVAEFRIRGVATNVPFLQAVLDDPEFRAGRITTAFIEQRPELLTARVSADRGTKILTYLADVTVNRPHGEHPIAVDPRDKLPALTRPGPASPPAGSRQRLLTLGPEGFAADLRARPQLGITDTTFRDAHQSLLATRVRTSDLLAVAPYVAASTPELLSVECWGGATYDVALRFLKEDPWERLAALREAIPNICLQMLLRGANTVGYTPYPTSVTTAFVDEAAATGVDIFRIFDALNNVEAMRPAIDAVRATGTGVAEVAMSYTGDLADPGENLYTLDYYLRLAEDIVSAGAHILAIKDMAGLLRAPAATRLVSALRREFDLPIHVHTHDTPGGQLATYLAAWEAGADAVDGASAPLAGTTSQPSLSAIVAATAHTPRDSGLDLAAVCDLEPYWESVRKVYAPFESGLPAPTGRVYHHEIPGGQLSNLRQQAIALGLGDRFEAIEHAYAAADRMLGRLIKVTPSSKVVGDLALALVGGGISAAGFAENPSAYDIPDSVVGFLRGELGDPAGGWPEPLRTRALEGRAAASQVNTLSDADEQTLRQPGRDRQELLNRLLFPAPTSEFEQHRNTYGDTSLLSTAQFFYGLRYGEEHSVELEPGVDLLTGLEAVSEPDDHGMRTVMAILNGQLRPVQVRDRAVGVEIPAAEKAEPGNDLHVAAPFAGVVTVAVAVGDDVEPGTTIATIEAMKMEAAITTRKGGTVARVAITSTAQVEAGDLVIELS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002907
EMBL· GenBank· DDBJ
AFR50938.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help