J9SRG0 · J9SRG0_9ACTN
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1129 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 117 | ATP (UniProtKB | ChEBI) | |||
Binding site | 200 | ATP (UniProtKB | ChEBI) | |||
Binding site | 235 | ATP (UniProtKB | ChEBI) | |||
Active site | 292 | ||||
Binding site | 535 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 607 | substrate | |||
Binding site | 705 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 734 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 736 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 869 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia
Accessions
- Primary accessionJ9SRG0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 705 | N6-carboxylysine | |||
Modified residue | 1095 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-449 | Biotin carboxylation | |||
Domain | 121-317 | ATP-grasp | |||
Region | 478-505 | Disordered | |||
Domain | 526-795 | Pyruvate carboxyltransferase | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,129
- Mass (Da)120,337
- Last updated2012-11-28 v1
- MD5 ChecksumF9AC5EA5ABAB4441B47543925002BB36
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002907 EMBL· GenBank· DDBJ | AFR50938.1 EMBL· GenBank· DDBJ | Genomic DNA |