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J9SLP5 · J9SLP5_9ACTN

  • Protein
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
  • Gene
    murE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site53UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site143-149ATP (UniProtKB | ChEBI)
Binding site185-186UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site212UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site220UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site422meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site446-449meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site506meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site510meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular FunctionUDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity
Biological Processcell division
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processregulation of cell shape

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
  • EC number
  • Alternative names
    • Meso-A2pm-adding enzyme
    • Meso-diaminopimelate-adding enzyme
    • UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    • UDP-MurNAc-tripeptide synthetase
    • UDP-N-acetylmuramyl-tripeptide synthetase

Gene names

    • Name
      murE
    • ORF names
      KTR9_3013

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KTR9
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia

Accessions

  • Primary accession
    J9SLP5

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue252N6-carboxylysine

Post-translational modification

Carboxylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, motif.

Type
IDPosition(s)Description
Domain48-96Mur ligase N-terminal catalytic
Domain141-352Mur ligase central
Region285-332Disordered
Domain373-508Mur ligase C-terminal
Motif446-449Meso-diaminopimelate recognition motif
Region533-555Disordered

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    555
  • Mass (Da)
    57,422
  • Last updated
    2012-11-28 v1
  • MD5 Checksum
    64F8E3B78DFD47FEEC3F9EEA40FF334B
MASRRRSQDPRGGPIRPSEVTPTLVSVLSTATGARLDLVGGADAESTVTGVTLRAQEVRPGDLFAALPGSSTHGARFADQAVAAGASAILTDPAGRAELARVLPPETAVAVLIHPAPRTVLGNVSARVYGNPSQRLKLIGVTGTSGKTTTSYLVEAALLAAGHSVGLIGTVETRVNGVAQPSSLTTPEAPTLQALLAAMLEDGVDIVVMEVSSHALALGRVDGAHFAIGAFTNLSQDHLDFHQTMRAYFDAKAQLFVPSAPTHAVRAVVCVDDEWGRRMAELARRPGESAHLQPVTVSTGPTPAHWHAGESSLDPDGSSRTPFSEPDGTHELRIPLPGRYNVANGLLAVAVAHAAGVSVPTALEAVASVSVPGRLQRIDRGQPFLALVDYAHKPGAVDAVLTTLRGQSTGRVAIVIGAGGDRDTGKRPLMGEAAARGAELVVVTDDNPRTEDPGAIRAEVLAGAYAVPVTERPRDAEPVREIGDRAAAVEAAIAWAQPGDIVLVAGKGHEAGQEINGVKHPFDDREVVARALESRAATSRATGTTRGPDAAAEER

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002907
EMBL· GenBank· DDBJ
AFR49648.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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