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J9SLM1 · J9SLM1_9ACTN

  • Protein
    Isoleucine--tRNA ligase
  • Gene
    ileS
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

110651002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site636ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      KTR9_2998

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KTR9
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Gordoniaceae > Gordonia

Accessions

  • Primary accession
    J9SLM1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, motif.

Type
IDPosition(s)Description
Region1-30Disordered
Domain37-667Aminoacyl-tRNA synthetase class Ia
Motif67-77'HIGH' region
Motif633-637'KMSKS' region
Domain715-855Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,065
  • Mass (Da)
    119,184
  • Last updated
    2012-11-28 v1
  • MD5 Checksum
    64F7913F3859D726B8CF400192670796
MSDATDPTQNARVYPKVDMTGGTGRSAPDFPSVEQRVLQYWDSDSTFAASIENRADAEEFVFYDGPPFANGLPHYGHLLTGYVKDLVPRYQTMRGKKVDRRFGWDTHGLPAELEAERQLGITDKSEIEKMGMAKFNEYCRDSVLRYTGEWRDYVTRQARWVDFDNDYKTLDLDFMESVMWAFKALYDKGLIYQGYRVLPYSWYEQTPLSNQESKLDDAYRMRQDPAVTVTMPLSVPDGPLSTLDGVNALIWTTTPWTLPSNLAIAVNPDVTYVHVRAGDGQEYLLAEALLGAYAKEIVDPEVLGTHRGVELKDLSYTPPFDFFAGHPNSHRVLLGDYVTTDSGTGIVHLAPAFGEEDMDLATANGIEVVQPLDPGGRFTAQVQPYEGLMVFDANPVIIKDLKETGRILRHETIEHSYPHSWRSGQPLIYMAVPSWFVAVTQFRDRMVELNKQIVWVPEHIRDGQFGKWLEGARDWNISRNRYWGAPLPVWVSDDPEFPRIDVYGSLDDLERDFGVRPDNLHRPFIDELTRPNPDDPSGKSTMRRVPEVLDCWFESGSMPFAQVHYPFENRDWFDGGDSAHAPAHNPGDFIVEYNGQTRGWFYTLHVLATALFDRPAFKTVAAHGIVLGDDGQKMSKSKRNYPDVNEVFDRDGSDAMRWFLMASPILRGGNLVVTERGIREGVRQALLPLWNAYSFLQLYAERPATWRTNSEHVLDRYILAKLATTRDSMTEALDIYDIAGACDAFREFVESLTNWYVRRSRARFWAGQDEDTDAFDTLYTVLEVACRLASPLLPLATEAIWRGLTGERSVHLTDWPTSDELPADADLVTAMDQVQAVCSTASSVRKANRLRVRLPLPGLTVASPTAGSLEPFVDLIKDEMNVKTVTLAADASEYGRYEIAVNARAAGPRLGKDVQRAIKAVKSGNWTVGDGADGSEVVVADGVELREGEFTRRLVAVEPDSTAELPGGNGLVVLDTTVTPELEAEGWAKDRVRELQDARRTLGLDVSDRIAVRFVVPAERLEWARTHADLIAGEVLAVRFDVTGDGDAGSIELGDGVTADVTKAG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002907
EMBL· GenBank· DDBJ
AFR49633.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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