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J7FIP8 · IDTP_CLAPA

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of paspalitrems, indole-diterpene (IDT) mycotoxins that are potent tremorgens in mammals (PubMed:23468653, PubMed:29457197, PubMed:32077051).
The geranylgeranyl diphosphate (GGPP) synthase idtG is proposed to catalyze the first step in IDT biosynthesis via catalysis of a series of iterative condensations of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), and farnesyl diphosphate (FPP), to form GGPP (Probable). Condensation of indole-3-glycerol phosphate with GGPP by the prenyltransferase idtC then forms 3-geranylgeranylindole (3-GGI) (Probable). Epoxidation of the two terminal alkenes of the geranylgeranyl moiety by the FAD-dependent monooxygenase idtM, and cyclization by the terpene cyclase idtB then leads to the production of paspaline (Probable). The cytochrome P450 monooxygenase idtP then catalyzes oxidative elimination of the pendant methyl group at C-12 of paspaline and generates the C-10 ketone to yield 13-desoxypaxilline (PubMed:32077051).
The cytochrome P450 monooxygenase idtQ may catalyze the C-13 oxidation of 13-desoxypaxilline to afford paxilline (Probable). Considering that both paspalicine and paxilline were detected in C.paspali, idtQ also catalyzes the formation of paspalinine from 13-desoxypaxilline via paspalicine as an intermediate (Probable). Finally, the alpha-prenyltransferase idtF prenylates paspalinine at the C-20 or the C-21 positions to yield paspalitrems A and C, respectively (PubMed:32077051).
The hydroxylation of paspalitrem A at C-32 by a still unknown oxidase affords paspalitrem B (Probable)

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site439Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase idtP
  • EC number
  • Alternative names
    • Indole-diterpene biosynthesis cluster protein P

Gene names

    • Name
      idtP

Organism names

  • Taxonomic identifier
  • Strain
    • RRC-1481
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Claviceps

Accessions

  • Primary accession
    J7FIP8

Phenotypes & Variants

Disruption phenotype

Abolishes the production of all paspalitrems, but accumulates paspaline.

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-20
ChainPRO_500379211721-498Cytochrome P450 monooxygenase idtP

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    498
  • Mass (Da)
    57,051
  • Last updated
    2012-10-31 v1
  • MD5 Checksum
    12B4C85C0EE88C4BCCE55C504162607C
MFLLHILAIGACLLWYFVRSDKRKQGSNIPTIRRWPALFPEFLDRLSYNSCAARLVENGYREHKDRPFRLLKMDMDLVVIPLKYAQEMRALTSDELDPLTAVFDDNVGKVTGILLDSQLHTHAIQRRLTPRLPNIIPAMMDELNYAFQQVIPSEAGERLQTSWVPINPYDMVLELSTRAAARLFVGEPICRDEVFLKTSAAYSRNIFDTIHVSRSLGHIITPYLGSLIPSVRQFHQQFEYIQNLVLGEIAHRKQHSEDNKADDFLQWCMELARTKEESTPTALAQRTVGILSMAVVHTSAMATTHLLFDMISNQELREQLRTEQKQVLKQGWMGITQQTMLDMKQLDSVMRESQRINPVGEFTFRRIVRKPITLSDGFQLHPGQQIAIAARCINTDGDVLPDAETFKPMRWMEKESAASTGFAHSSDSNLHFGLGRYACPGRFLASYMIKAIISRVLFDYEFKLQDDSAAGRPPNLIHGDKIFPSRDVTVLFRRRHDE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JN613321
EMBL· GenBank· DDBJ
AFO85418.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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