J4KLK4 · MT1_BEAB2
- ProteinMethyltransferase 1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids249 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Methyltransferase; part of the pathway that mediates the biosynthesis of tenellin-type 2-pyridones, iron-chelating compounds involved in iron stress tolerance, competition with the natural competitor fungus Metarhizium robertsii and insect hosts infection (PubMed:34903054).
Methylates pyridovericin-N-O-(beta-D-glucopyranoside) produced by the UDP-glucosyltransferase GT1 to yield pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP) (PubMed:34903054).
The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC. These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D. The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively. The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively. The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP). Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).
Methylates pyridovericin-N-O-(beta-D-glucopyranoside) produced by the UDP-glucosyltransferase GT1 to yield pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP) (PubMed:34903054).
The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC. These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D. The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively. The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively. The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP). Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).
Pathway
Secondary metabolite biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | methyltransferase activity | |
Biological Process | methylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethyltransferase 1
- EC number
- Short namesMT1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Cordycipitaceae > Beauveria
Accessions
- Primary accessionJ4KLK4
Proteomes
Phenotypes & Variants
Disruption phenotype
Impairs the production of detectable pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455691 | 1-249 | Methyltransferase 1 | |||
Sequence: MALVEKIQLTDDFSVYANPAAKLEVEFIHKEIFIDKCYDVAPFPDDSFIVDAGGNIGMFTLYMKRKYPQSTILAFEPAPATFSTFQRNMELHNVSGVQAHQCGLGREDASLALTFYPQMPGNSTLYAEDKTNQMKSVDQNHPIAKLMQETHEVQVDVKRLSDFLGEVPNLKRVNLLKVDVEGAEMDVLRGLDDEHWDLIDNVVVELCDSKGDFATAKTLLESKGFAVAVERPDWAPPDLKMYMLIAKRN |
Expression
Induction
Expression is positively regulated by the cluster-specific transcription factor tenR and is induced during cocultures with the natural competitor fungus Metarhizium robertsii.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length249
- Mass (Da)28,021
- Last updated2012-10-31 v1
- Checksum9F9DD0D2091CA10A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH725187 EMBL· GenBank· DDBJ | EJP62359.1 EMBL· GenBank· DDBJ | Genomic DNA |