J4KLK4 · MT1_BEAB2

Function

function

Methyltransferase; part of the pathway that mediates the biosynthesis of tenellin-type 2-pyridones, iron-chelating compounds involved in iron stress tolerance, competition with the natural competitor fungus Metarhizium robertsii and insect hosts infection (PubMed:34903054).
Methylates pyridovericin-N-O-(beta-D-glucopyranoside) produced by the UDP-glucosyltransferase GT1 to yield pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP) (PubMed:34903054).
The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC. These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D. The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively. The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively. The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP). Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionmethyltransferase activity
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methyltransferase 1
  • EC number
  • Short names
    MT1
  • Alternative names
    • Sordarin/hypoxysordarin biosynthesis cluster protein D

Gene names

    • ORF names
      BBA_08685

Organism names

Accessions

  • Primary accession
    J4KLK4

Proteomes

Phenotypes & Variants

Disruption phenotype

Impairs the production of detectable pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004556911-249Methyltransferase 1

Expression

Induction

Expression is positively regulated by the cluster-specific transcription factor tenR and is induced during cocultures with the natural competitor fungus Metarhizium robertsii.

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the FkbM methyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    249
  • Mass (Da)
    28,021
  • Last updated
    2012-10-31 v1
  • Checksum
    9F9DD0D2091CA10A
MALVEKIQLTDDFSVYANPAAKLEVEFIHKEIFIDKCYDVAPFPDDSFIVDAGGNIGMFTLYMKRKYPQSTILAFEPAPATFSTFQRNMELHNVSGVQAHQCGLGREDASLALTFYPQMPGNSTLYAEDKTNQMKSVDQNHPIAKLMQETHEVQVDVKRLSDFLGEVPNLKRVNLLKVDVEGAEMDVLRGLDDEHWDLIDNVVVELCDSKGDFATAKTLLESKGFAVAVERPDWAPPDLKMYMLIAKRN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JH725187
EMBL· GenBank· DDBJ
EJP62359.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp