J3P745 · J3P745_GAET3
- ProteinPyruvate carboxylase
- Gene20349800
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1198 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 162 | ATP (UniProtKB | ChEBI) | |||
Binding site | 246 | ATP (UniProtKB | ChEBI) | |||
Binding site | 281 | ATP (UniProtKB | ChEBI) | |||
Active site | 338 | ||||
Binding site | 591 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 663 | substrate | |||
Binding site | 759 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 789 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 791 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 924 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Magnaporthales > Magnaporthaceae > Gaeumannomyces
Accessions
- Primary accessionJ3P745
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 759 | N6-carboxylysine | |||
Modified residue | 1161 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 44-496 | Biotin carboxylation | |||
Domain | 166-363 | ATP-grasp | |||
Domain | 582-850 | Pyruvate carboxyltransferase | |||
Domain | 1120-1195 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,198
- Mass (Da)131,649
- Last updated2012-10-03 v1
- MD5 Checksum9CB28C2D322EEF163ACE26F224DAFD8C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GL385399 EMBL· GenBank· DDBJ | EJT72476.1 EMBL· GenBank· DDBJ | Genomic DNA |