J3LMJ1 · J3LMJ1_ORYBR
- ProteinArginine biosynthesis bifunctional protein ArgJ, chloroplastic
- Gene102718722
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids465 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.
Catalytic activity
- L-glutamate + N2-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N2-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N2-acetyl-L-ornithine from L-glutamate: step 1/4.
Features
Showing features for site, binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 170 | Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole | ||||
Sequence: T | ||||||
Site | 171 | Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole | ||||
Sequence: G | ||||||
Binding site | 209 | substrate | ||||
Sequence: T | ||||||
Binding site | 235 | substrate | ||||
Sequence: K | ||||||
Site | 245-246 | Cleavage; by autolysis | ||||
Sequence: AT | ||||||
Active site | 246 | Nucleophile | ||||
Sequence: T | ||||||
Binding site | 246 | substrate | ||||
Sequence: T | ||||||
Binding site | 333 | substrate | ||||
Sequence: E | ||||||
Binding site | 460 | substrate | ||||
Sequence: N | ||||||
Binding site | 465 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | acetyl-CoA:L-glutamate N-acetyltransferase activity | |
Molecular Function | glutamate N-acetyltransferase activity | |
Molecular Function | methione N-acyltransferase activity | |
Biological Process | arginine biosynthetic process | |
Biological Process | ornithine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameArginine biosynthesis bifunctional protein ArgJ, chloroplastic
- Cleaved into 2 chains
Including 2 domains:
- Recommended nameGlutamate N-acetyltransferase
- EC number
- Short namesGAT
- Alternative names
- Recommended nameAmino-acid acetyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza
Accessions
- Primary accessionJ3LMJ1
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5023261889 | 1-245 | Arginine biosynthesis bifunctional protein ArgJ alpha chain | |||
Sequence: MPPPSLLLLHPRAPLPHRRSSFRMSSPPPGRVVCAAAASTEGFVSAAPILLPDGPWKQVEGGVTAAKGFKAAGIYGGLRAKGEKPDLALVACDVDATVAGAFTTNVVAAAPVLYCKRVLSSSKTARAVLINAGQANAATGDAGYQDAVDSADAVAKLLNVSTNDILIQSTGVIGQRIKKEALVNSLPRLVGSLSSSIQGANSAAVAITTTDLVSKSIAVQTEIGGVPIKIGGMAKGSGMIHPNMA | ||||||
Chain | PRO_5023261888 | 246-465 | Arginine biosynthesis bifunctional protein ArgJ beta chain | |||
Sequence: TMLGVLTTDAQVSSDVWREMVRTSVSRSFNQITVDGDTSTNDCVIAMASGLSGLSSILTHDSTEAQQFQACLDAVMQGLAKSIAWDGEGATCLIEVTVTGANNEVEAAKIARSVASSSLVKAAVFGRDPNWGRIACSVGYSGIQFDADQLDICLGDTPLMKNGQPLPFDRSAASKYLKDAGDIHGTVNIDVSVGRGEGSGKAWGCDLSYKYVEINAEYTT |
Keywords
- PTM
Interaction
Structure
Sequence
- Sequence statusComplete
- Length465
- Mass (Da)47,958
- Last updated2012-10-03 v1
- Checksum86622212B920E3FF
Keywords
- Technical term