J3JXR6 · J3JXR6_DENPD

Function

function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Miscellaneous

Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site120Mn2+ 1 (UniProtKB | ChEBI)
Binding site123Mn2+ 1 (UniProtKB | ChEBI)
Binding site123Mn2+ 2 (UniProtKB | ChEBI)
Binding site227-231GMP (UniProtKB | ChEBI)
Binding site228Mn2+ 1 (UniProtKB | ChEBI)
Binding site260Mn2+ 2 (UniProtKB | ChEBI)
Binding site354Mn2+ 2 (UniProtKB | ChEBI)
Binding site354-355GMP (UniProtKB | ChEBI)
Binding site403-406GMP (UniProtKB | ChEBI)
Binding site410GMP (UniProtKB | ChEBI)
Active site429GMP-histidine intermediate
Binding site429-432GMP (UniProtKB | ChEBI)
Binding site505GMP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (GTP) activity
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-splicing ligase RtcB homolog
  • EC number
  • Alternative names
    • 3'-phosphate/5'-hydroxy nucleic acid ligase

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Coleoptera > Polyphaga > Cucujiformia > Curculionidae > Scolytinae > Dendroctonus

Accessions

  • Primary accession
    J3JXR6

Subcellular Location

Interaction

Subunit

Catalytic component of the tRNA-splicing ligase complex.

Family & Domains

Sequence similarities

Belongs to the RtcB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    56,045
  • Last updated
    2012-10-03 v1
  • Checksum
    815980DA73E21300
MVVRQYNEELAYLEKINNHCWRIKKGFQPNMRVEGVFYVNQTLEKLMLEELRNSCRPGMVGGFLPGVKQIANVAALPGIVGKSIGLPDIHSGYGFAIGNMAAFDMNDPESIVSPGGVGFDINCGVRLLRTNLFEKDVQPVKEQLAQSLFDHIPVGVGSKGIIPMNAKDLEEALEMGMDWSLREGYIWAEDKEHCEEYGRMLNADPSKVSMRAKKRGLPQLGTLGAGNHYAEIQVVDEIYDKWAACKMGIEEKGQVCVMIHSGSRGFGHQVATDALVQMEKAMKRDKIETNDRQLACARINSVEGQDYLKSMAAAANFAWVNRSSMTFLTRQAFAKQFDTTPDDLDMHVIYDVSHNIAKVEEHIVDGKQKTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMVTCSYVLTGTEQGMLETFGSTCHGAGRALSRAKSRRNIDYTDVLKRMEDMNIAIRVASPKLVMEEAPESYKNVTDVVNTCHAAGISKKAIKLRPIAVIKG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BT128037
EMBL· GenBank· DDBJ
AEE62998.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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