J3H6M3 · J3H6M3_9PSED
- ProteinBifunctional polymyxin resistance protein ArnA
- GenearnA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids663 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.
Catalytic activity
- UDP-4-amino-4-deoxy-beta-L-arabinose + (6R)-10-formyltetrahydrofolate = UDP-4-deoxy-4-formamido-beta-L-arabinose + (6S)-5,6,7,8-tetrahydrofolate + H+
Pathway
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3.
Features
Showing features for site, active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 104 | Transition state stabilizer | |||
Active site | 106 | Proton donor; for formyltransferase activity | |||
Binding site | 116 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 138-142 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 142 | Raises pKa of active site His | |||
Binding site | 350 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 371-372 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 396 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 401 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 435-436 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Active site | 437 | Proton acceptor; for decarboxylase activity | |||
Binding site | 463 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 494 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 528-537 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 615 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Active site | 621 | Proton donor; for decarboxylase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carboxy-lyase activity | |
Molecular Function | UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity | |
Molecular Function | UDP-glucuronic acid dehydrogenase activity | |
Biological Process | lipid A biosynthetic process | |
Biological Process | lipopolysaccharide biosynthetic process | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional polymyxin resistance protein ArnA
Including 2 domains:
- Recommended nameUDP-4-amino-4-deoxy-L-arabinose formyltransferase
- EC number
- Alternative names
- Recommended nameUDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionJ3H6M3
Proteomes
Interaction
Subunit
Homohexamer, formed by a dimer of trimers.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-307 | Formyltransferase ArnAFT | |||
Domain | 27-176 | Formyl transferase N-terminal | |||
Domain | 204-284 | Formyl transferase C-terminal | |||
Region | 317-663 | Dehydrogenase ArnADH | |||
Domain | 321-568 | NAD-dependent epimerase/dehydratase | |||
Sequence similarities
In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.
In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length663
- Mass (Da)73,669
- Last updated2012-10-03 v1
- ChecksumF17F758070023874
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AKJG01000106 EMBL· GenBank· DDBJ | EJM86135.1 EMBL· GenBank· DDBJ | Genomic DNA |