J3BIN2 · J3BIN2_9PSED

  • Protein
    tRNA sulfurtransferase
  • Gene
    thiI
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site185-186ATP (UniProtKB | ChEBI)
Binding site267ATP (UniProtKB | ChEBI)
Binding site289ATP (UniProtKB | ChEBI)
Binding site298ATP (UniProtKB | ChEBI)
Active site457Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      PMI33_03420

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GM67
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    J3BIN2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain63-167THUMP
Domain405-481Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    484
  • Mass (Da)
    54,424
  • Last updated
    2012-10-03 v1
  • Checksum
    989C3CB4F81796F6
MKLIVKVFPEITIKSRPVRMRFIRQLAKNIRAVLRDLDPAVVVNGVWDNLELETRVSDPKALKEMGERLSCMPGVAHFLQIDEYPLGDFDDIVEKCKQHYGDALAGKIFSVRCKRAGKHPFSSIDIEKYVGSQLRRQCGAAGIDLKKPEIEVRIEVRDQRLFVIHSQHNGIGGYPLGALEQTLVLMSGGFDSTVAAYQIMRRGLMAHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVLFVSVPFEEVLGEILGKVDNSHMGVVLKRMMLRAATQIADRLQIEALVTGEAISQVSSQTLPNLNVIDCVTEKLVLRPLIASHKQDIIDLANEIGTADFARHMPEYCGVISVNPKTAAKRHRVEHEEKEFDMAVLERALENAKLVPIDRVIDELGQDLQIEEVSEALAGQIIIDIRHPDAAEDEPLELGGIEVQTMPFYALNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRVYRPS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AKJH01000066
EMBL· GenBank· DDBJ
EJM86188.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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