J3BIN2 · J3BIN2_9PSED
- ProteintRNA sulfurtransferase
- GenethiI
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids484 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
Catalytic activity
- [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H+ = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate
- [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + L-cysteinyl-[cysteine desulfurase] + A + AMP + H+
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 185-186 | ATP (UniProtKB | ChEBI) | |||
Binding site | 267 | ATP (UniProtKB | ChEBI) | |||
Binding site | 289 | ATP (UniProtKB | ChEBI) | |||
Binding site | 298 | ATP (UniProtKB | ChEBI) | |||
Active site | 457 | Cysteine persulfide intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uracil-4 sulfurtransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiamine diphosphate biosynthetic process | |
Biological Process | thiazole biosynthetic process | |
Biological Process | tRNA 4-thiouridine biosynthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA sulfurtransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionJ3BIN2
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length484
- Mass (Da)54,424
- Last updated2012-10-03 v1
- Checksum989C3CB4F81796F6
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AKJH01000066 EMBL· GenBank· DDBJ | EJM86188.1 EMBL· GenBank· DDBJ | Genomic DNA |