I9L241 · I9L241_LACPE

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site41-45NAD+ (UniProtKB | ChEBI)
Binding site90-91NAD+ (UniProtKB | ChEBI)
Binding site120NAD+ (UniProtKB | ChEBI)
Active site122N6-AMP-lysine intermediate
Binding site143NAD+ (UniProtKB | ChEBI)
Binding site177NAD+ (UniProtKB | ChEBI)
Binding site293NAD+ (UniProtKB | ChEBI)
Binding site317NAD+ (UniProtKB | ChEBI)
Binding site411Zn2+ (UniProtKB | ChEBI)
Binding site414Zn2+ (UniProtKB | ChEBI)
Binding site429Zn2+ (UniProtKB | ChEBI)
Binding site434Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      KCA1_0912

Organism names

Accessions

  • Primary accession
    I9L241

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain597-679BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    679
  • Mass (Da)
    74,765
  • Last updated
    2012-10-03 v1
  • Checksum
    03BD8DD468C0486C
MADEPNTMTVSQAAEEATTLRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELERQFPQLVTPDSPTQLVGGTVKSGFDKVTHEIPMLSLGDVFSQAELQEFADRLEQNVGHAVDYNCELKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIKSIPQRLTKPLTIDVRGECYMPKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLKARTQSDMLTEFADLGFAINPDFKVAHSMSEVFDYIDQYQNERPELAYGIDGIVIKANPLPLQRSLGATVKVPRWAIAYKFPPDEQPTTLLDVEWTVGRTGVVTPTAVMEPVQLAGTTVARASLHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLKQRPKDAQPLEIPTDCPSCGAPLVHLEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYTNHLVADVADLYRLTKDQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEALMAASQEEIAAIDSMGDVIANAVVQYFESDEVHTLISELQAVKVNTTYQGPSATVAEDSNSWFAGKRVVLTGKLESFTRPEATQWLQAHGATVTGSVSKKTDLVIAGSDAGSKLQKAQQLDITVWDEARFSETMREDTQA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AKAO01000029
EMBL· GenBank· DDBJ
EIW14354.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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