I9L241 · I9L241_LACPE
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids679 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 41-45 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 90-91 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 120 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 122 | N6-AMP-lysine intermediate | |||
Binding site | 143 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 177 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 293 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 317 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 411 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 414 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 429 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 434 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lactiplantibacillus
Accessions
- Primary accessionI9L241
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 597-679 | BRCT | |||
Sequence similarities
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length679
- Mass (Da)74,765
- Last updated2012-10-03 v1
- Checksum03BD8DD468C0486C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AKAO01000029 EMBL· GenBank· DDBJ | EIW14354.1 EMBL· GenBank· DDBJ | Genomic DNA |