I7FPM8 · I7FPM8_MYCS2

Function

function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site157Nucleophile; methyl group acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmethylated-DNA-[protein]-cysteine S-methyltransferase activity
Biological ProcessDNA alkylation repair
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methylated-DNA--protein-cysteine methyltransferase
  • EC number
  • Alternative names
    • 6-O-methylguanine-DNA methyltransferase
      (MGMT
      )
    • O-6-methylguanine-DNA-alkyltransferase

Gene names

    • Ordered locus names
      MSMEI_4216

Organism names

Accessions

  • Primary accession
    I7FPM8

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain25-100Methylguanine DNA methyltransferase ribonuclease-like
Domain107-185Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding

Sequence similarities

Belongs to the MGMT family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    187
  • Mass (Da)
    20,207
  • Last updated
    2017-05-10 v1
  • Checksum
    0B94D3B1CEAEF2BC
MNDTLIRLHRRLEQSATTDGLLDVAYTTVDTPVGPLLLAATEIGLVRVAFATEDHDAVLDTLAQRLSPRILRAPQRLADAARQIDEYFAGARTQFDVALDHSLSHGFRQLVQRRLSQIPYGHTMSYKGVAEIVGNPNAVRAVGSACSTNPLPVVVPCHRVLRSDGSLGGYIGGLDAKTALLELERAA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001663
EMBL· GenBank· DDBJ
AFP40673.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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