I7C6E8 · C7A52_PANGI

Function

function

Component of the oleanane-type triterpene saponins (e.g. ginsenosides or panaxosides) biosynthetic pathway (PubMed:29378087).
Catalyzes the carboxylation of beta-amyrin at the C-28 position to form oleanolic acid during ginsenoside biosynthesis, a class of tetracyclic triterpenoid saponins

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site428Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
Molecular Functionursolic aldehyde 28-monooxygenase activity
Molecular Functionuvaol dehydrogenase activity
Biological Processpentacyclic triterpenoid metabolic process
Biological Processsterol metabolic process
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-amyrin 28-monooxygenase
  • EC number
  • Alternative names
    • Beta-amyrin 28-oxidase
    • Cytochrome P450 CYP716A52v2
    • Oleanic acid synthase
      (PgOAS
      )

Gene names

    • Name
      CYP716A52v2
    • Synonyms
      OAS

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > campanulids > Apiales > Araliaceae > Panax

Accessions

  • Primary accession
    I7C6E8

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane4-24Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004258811-481Beta-amyrin 28-monooxygenase

Expression

Tissue specificity

Mostly expressed in roots, and, to a lower extent, in stems and leaves (PubMed:30577538).
Accumulates only in the rhizome of plants (PubMed:22875608).

Induction

Expression is not affected by methyl jasmonate (MeJA) treatment (PubMed:22875608).
Influenced in roots and leaves by relative humidity and soil water potential (PubMed:30577538).

Developmental stage

Progressive increase in roots from the leaf opened stage to the green fruit, red fruit and root growth stages, respectively.

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    481
  • Mass (Da)
    53,955
  • Last updated
    2012-10-03 v1
  • Checksum
    728979F41FD9244F
MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFPLPPGKTGWPIIGESYEFLSTGWKGYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAKSYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKLVGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSACTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFVPFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPHKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JX036032
EMBL· GenBank· DDBJ
AFO63032.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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