I7C6E8 · C7A52_PANGI
- ProteinBeta-amyrin 28-monooxygenase
- GeneCYP716A52v2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids481 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the oleanane-type triterpene saponins (e.g. ginsenosides or panaxosides) biosynthetic pathway (PubMed:29378087).
Catalyzes the carboxylation of beta-amyrin at the C-28 position to form oleanolic acid during ginsenoside biosynthesis, a class of tetracyclic triterpenoid saponins
Catalyzes the carboxylation of beta-amyrin at the C-28 position to form oleanolic acid during ginsenoside biosynthesis, a class of tetracyclic triterpenoid saponins
Catalytic activity
- beta-amyrin + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4 H+ + 4 H2O + oleanolate + 3 oxidized [NADPH--hemoprotein reductase]
Cofactor
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen | |
Molecular Function | ursolic aldehyde 28-monooxygenase activity | |
Molecular Function | uvaol dehydrogenase activity | |
Biological Process | pentacyclic triterpenoid metabolic process | |
Biological Process | sterol metabolic process | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBeta-amyrin 28-monooxygenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > campanulids > Apiales > Araliaceae > Panax
Accessions
- Primary accessionI7C6E8
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 4-24 | Helical | ||||
Sequence: FYVPLLSLFVLFISLSFHFLF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000425881 | 1-481 | Beta-amyrin 28-monooxygenase | |||
Sequence: MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFPLPPGKTGWPIIGESYEFLSTGWKGYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAKSYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKLVGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSACTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFVPFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPHKA |
Expression
Tissue specificity
Mostly expressed in roots, and, to a lower extent, in stems and leaves (PubMed:30577538).
Accumulates only in the rhizome of plants (PubMed:22875608).
Accumulates only in the rhizome of plants (PubMed:22875608).
Induction
Expression is not affected by methyl jasmonate (MeJA) treatment (PubMed:22875608).
Influenced in roots and leaves by relative humidity and soil water potential (PubMed:30577538).
Influenced in roots and leaves by relative humidity and soil water potential (PubMed:30577538).
Developmental stage
Progressive increase in roots from the leaf opened stage to the green fruit, red fruit and root growth stages, respectively.
Structure
Sequence
- Sequence statusComplete
- Length481
- Mass (Da)53,955
- Last updated2012-10-03 v1
- Checksum728979F41FD9244F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JX036032 EMBL· GenBank· DDBJ | AFO63032.1 EMBL· GenBank· DDBJ | mRNA |