I6Y204 · DACB2_MYCTU

Function

function

Probably cleaves the terminal D-Ala-D-Ala dipeptide of the peptidoglycan stem peptide (Probable). Shows significant D,D-carboxypeptidase activity in vitro (PubMed:22906310).
Acts on the synthetic penta-peptide substrate Penta-DAP (L-Ala-gamma-D-Gln-DAP-D-Ala-D-Ala). Shows also weak activity on Penta-Lys (L-Ala-gamma-Glu-L-Lys-D-Ala-D-Ala) (PubMed:22906310).
The catalytic domain binds weakly to peptidoglycan in vitro (PubMed:25551456).
Plays an important role in the maintenance of colony morphology and cell wall permeability and integrity (PubMed:25467937).

Miscellaneous

Overexpression in Mycobacterium smegmatis results in reduced growth, an altered colony morphology, and a defect in sliding motility and biofilm formation. Overexpression of the S69C mutant shows similar results, indicating that the effects produced are independent of protein's penicillin binding function.

Activity regulation

Inhibited by the beta-lactam antibiotic meropenem (PubMed:22906310).
Inhibited by the non-specific inhibitor phenylmethylsulfonyl fluoride (PMSF) (Probable)

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site69Acyl-ester intermediate
Active site72Proton acceptor
Active site124

GO annotations

AspectTerm
Cellular Componentperiplasmic space
Molecular Functionserine-type D-Ala-D-Ala carboxypeptidase activity
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processproteolysis
Biological Processregulation of cell shape

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    D-alanyl-D-alanine carboxypeptidase DacB2
  • EC number
  • Short names
    D,D-carboxypeptidase DacB2
    ; DD-carboxypeptidase
    ; DD-peptidase

Gene names

    • Name
      dacB2
    • Ordered locus names
      Rv2911

Organism names

Accessions

  • Primary accession
    I6Y204

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Deletion of the gene results in reduced growth in acidic medium under low oxygen conditions. The mutant shows better intracellular growth and survival inside THP-1 cells compared to wild-type and complemented strains. The colony morphology and antibiotic sensitivity of mutant and wild-type strains are similar (PubMed:22138550).
The double knockout mutant pknI/dacB2 shows smoother colony morphology on solid agar and exhibits defective biofilm and cord formation. Double mutant is hypersensitive to cell wall damaging agents such as lysozyme, malachite green, ethidium bromide and to isoniazid, a first line anti-TB drug (PubMed:25467937).

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_501024496623-291D-alanyl-D-alanine carboxypeptidase DacB2

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the peptidase S11 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    291
  • Mass (Da)
    29,747
  • Last updated
    2012-10-03 v1
  • Checksum
    BE6282F8DDAFB3B8
MRKLMTATAALCACAVTVSAGAAWADADVQPAGSVPIPDGPAQTWIVADLDSGQVLAGRDQNVAHPPASTIKVLLALVALDELDLNSTVVADVADTQAECNCVGVKPGRSYTARQLLDGLLLVSGNDAANTLAHMLGGQDVTVAKMNAKAATLGATSTHATTPSGLDGPGGSGASTAHDLVVIFRAAMANPVFAQITAEPSAMFPSDNGEQLIVNQDELLQRYPGAIGGKTGYTNAARKTFVGAAARGGRRLVIAMMYGLVKEGGPTYWDQAATLFDWGFALNPQASVGSL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP45713.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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