I6WC04 · I6WC04_9CAUD

  • Protein
    RNA ligase 1
  • Gene
    rnlA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in countering a host defense mechanism which, following viral infection, activates the host anticodon nuclease and shuts off viral translation. Repairs 5'-PO4 and 3'-OH groups in the cleaved host tRNA.

Catalytic activity

  • ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.
    EC:6.5.1.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions that perform the catalytic activity via a two-metal mechanism. One of the catalytic Mg2+, which is coordinated by 5 water molecules, engages the lysine nucleophile and the ATP alpha phosphate while the Mg2+ orients the PPi leaving group.

Features

Showing features for binding site, active site, site.

139250100150200250300350
TypeIDPosition(s)Description
Binding site37ATP (UniProtKB | ChEBI)
Binding site54ATP (UniProtKB | ChEBI)
Binding site75ATP (UniProtKB | ChEBI)
Active site111N6-AMP-lysine intermediate
Binding site176ATP (UniProtKB | ChEBI)
Site176Essential for RNA ligase activity
Binding site261ATP (UniProtKB | ChEBI)
Binding site263ATP (UniProtKB | ChEBI)
Site267Essential for RNA ligase activity
Binding site293Mg2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionRNA ligase (ATP) activity
Biological ProcessRNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA ligase 1
  • EC number
  • Alternative names
    • Rnl1

Gene names

    • Name
      rnlA
    • ORF names
      CC2_136

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Emmerichvirinae > Ceceduovirus > Ceceduovirus cc2

Accessions

  • Primary accession
    I6WC04

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain52-267T4 RNA ligase 1-like N-terminal
Domain274-391T4 RNA ligase 1 C-terminal

Sequence similarities

Belongs to the Tequatrovirus RNA ligase 1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    392
  • Mass (Da)
    45,609
  • Last updated
    2012-10-03 v1
  • Checksum
    436AFB6E0981EE07
MKELYNNLLKLTEEHGDCFFFRDHWSSIGNHFRVFSYHIAGLTQWMLPDALECRGIMFELINGEPYRIASRPMEKFFNLAERQAWNLTNSGVVGLDKLELDYSNIDRYEDKADGSLMSSYHFIDPEDDNRINYMLKSKTSINSDQANDANRWLVNHTDLLDFIIDCEEAGYTVNLEWCSPKNQIVIMYPEESLKILNVRHRDTGEYYSNNSLIRSPVFRKYAVDQFMFEEGTDVNTAISNMYNETGIEGYILVMRDGSRVKIKTTSYVARHKLKDSITNNKDLVIAVAQGVSDDLRQLFLDDSLSLTKIQEFEDHVVSVAGSTYTKIREAHKACAGMERREYAISMQNTFKQDRMFFNIVMKMFQAPDLEVMPEIMSVIIKYPDEFVPTKWK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JX123262
EMBL· GenBank· DDBJ
AFN39495.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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