I4YGH1 · I4YGH1_WALMC
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1188 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- ATP + hydrogencarbonate + pyruvate = ADP + H+ + oxaloacetate + phosphate
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 152 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 236 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 271 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 328 | |||||
Sequence: R | ||||||
Binding site | 583 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 655 | substrate | ||||
Sequence: R | ||||||
Binding site | 752 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 782 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 784 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 917 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Wallemiomycotina > Wallemiomycetes > Wallemiales > Wallemiaceae > Wallemia
Accessions
- Primary accessionI4YGH1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 752 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1153 | N6-biotinyllysine | ||||
Sequence: K |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-29 | Disordered | ||||
Sequence: MNPAFASEFRESSSVPSTPGPTSIHDIRQ | ||||||
Domain | 36-486 | Biotin carboxylation | ||||
Sequence: TGKRLLVANRGEIAVRIIRSVHELSMQTVSIYSHEDRLSSHRYKSDEAYQVGAGKPPVSAYLDQDDIVRIAKEHKVDLIHPGYGFLSENAEFARKCAENGITFVGPTPEAIDGLGDKTKARKLAMESNVPVVPGTKGAIDSYDQANAFIEEYGFPVIIKAAMGGGGRGMRVVRSQGEFEEAFGRAVSEAKSAFGDGTVFVERFLDHPRHIEVQLLADSHGNVIHLFERDCSVQRRHQKVVEIAPAPNLSDEVRNSILNDAKTLAKAIGYRNAGTAEFLVDQMGRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIACGASLAQLGLTQDKITKRGSAIQCRITTEDPSVGFAPDTGRIEVYRSAGGNGIRLDASNGYAGAVITPHYDSLLVKVSVRGQSYDLARRKMLRALVEFRIRGVKTNIPFLFRLLTHEVFVAGNTWTTFIDDSPE | ||||||
Domain | 156-353 | ATP-grasp | ||||
Sequence: RKLAMESNVPVVPGTKGAIDSYDQANAFIEEYGFPVIIKAAMGGGGRGMRVVRSQGEFEEAFGRAVSEAKSAFGDGTVFVERFLDHPRHIEVQLLADSHGNVIHLFERDCSVQRRHQKVVEIAPAPNLSDEVRNSILNDAKTLAKAIGYRNAGTAEFLVDQMGRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAC | ||||||
Domain | 574-843 | Pyruvate carboxyltransferase | ||||
Sequence: CLIMDTTWRDAHQSLLATRLRTVDMANIAKETSHALANAYSLECWGGATFDVAMRFLYEDPWQRLRTLRQLVPNIPFQALVRGANGVGYTSYPDNVIYDFSKKAVENGLDIFRVFDSLNYIENMKLGIDAAKKAGGVVEATVCYTGDVASPNEKKYTLEYYVNFVQELVDCGIHVLGIKDMAGLLKPHAATLLIGAIRQRFPELPIHVHSHDTAGISAASMLACAQAGADVVDVAIDSMSGLTSQPSMGAVVSALEQTGLGAGIRHEDIQ | ||||||
Domain | 1109-1187 | Lipoyl-binding | ||||
Sequence: VQREKATREPGSVGAPMGGVIIELRVHEGQEVKEGDPLAVISAMKMENSISAPASGKIARVVVHESDSLSAGDLICEIA |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,188
- Mass (Da)129,956
- Last updated2012-09-05 v1
- Checksum57C7F38C88738C8C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH668226 EMBL· GenBank· DDBJ | EIM23063.1 EMBL· GenBank· DDBJ | Genomic DNA |