I4YGH1 · I4YGH1_WALMC

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site152ATP (UniProtKB | ChEBI)
Binding site236ATP (UniProtKB | ChEBI)
Binding site271ATP (UniProtKB | ChEBI)
Active site328
Binding site583Mn2+ (UniProtKB | ChEBI)
Binding site655substrate
Binding site752Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site782Mn2+ (UniProtKB | ChEBI)
Binding site784Mn2+ (UniProtKB | ChEBI)
Binding site917substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • ORF names
      WALSEDRAFT_27737

Organism names

Accessions

  • Primary accession
    I4YGH1

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue752N6-carboxylysine
Modified residue1153N6-biotinyllysine

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-29Disordered
Domain36-486Biotin carboxylation
Domain156-353ATP-grasp
Domain574-843Pyruvate carboxyltransferase
Domain1109-1187Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,188
  • Mass (Da)
    129,956
  • Last updated
    2012-09-05 v1
  • Checksum
    57C7F38C88738C8C
MNPAFASEFRESSSVPSTPGPTSIHDIRQKSGQPLTGKRLLVANRGEIAVRIIRSVHELSMQTVSIYSHEDRLSSHRYKSDEAYQVGAGKPPVSAYLDQDDIVRIAKEHKVDLIHPGYGFLSENAEFARKCAENGITFVGPTPEAIDGLGDKTKARKLAMESNVPVVPGTKGAIDSYDQANAFIEEYGFPVIIKAAMGGGGRGMRVVRSQGEFEEAFGRAVSEAKSAFGDGTVFVERFLDHPRHIEVQLLADSHGNVIHLFERDCSVQRRHQKVVEIAPAPNLSDEVRNSILNDAKTLAKAIGYRNAGTAEFLVDQMGRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIACGASLAQLGLTQDKITKRGSAIQCRITTEDPSVGFAPDTGRIEVYRSAGGNGIRLDASNGYAGAVITPHYDSLLVKVSVRGQSYDLARRKMLRALVEFRIRGVKTNIPFLFRLLTHEVFVAGNTWTTFIDDSPELFKLVTSQNRAQKLLTYLGDVVVNGSSIKGQVGEPGLKSEIVVPKLPSRDGSADAHDTSKPCTSGWRNVILEKGPEGFAKAIRDYKGCLIMDTTWRDAHQSLLATRLRTVDMANIAKETSHALANAYSLECWGGATFDVAMRFLYEDPWQRLRTLRQLVPNIPFQALVRGANGVGYTSYPDNVIYDFSKKAVENGLDIFRVFDSLNYIENMKLGIDAAKKAGGVVEATVCYTGDVASPNEKKYTLEYYVNFVQELVDCGIHVLGIKDMAGLLKPHAATLLIGAIRQRFPELPIHVHSHDTAGISAASMLACAQAGADVVDVAIDSMSGLTSQPSMGAVVSALEQTGLGAGIRHEDIQALNLYWTQCRILYQCFEANVRSSDSGVFDHEMPGGQYTNLMFQAQQLGLGTQWTEIKQKYIEANELCGNIVKVTPSSKVVGDLAQFMVSNQLSKKDVEERADKLDFPNSVIEFFQGYLGQPVGGFPEPLRTKIIRDKPRIDGRPGIGMTPVDLKQVKKDLVQRFGKHITDADVSSYCLYPKVFEEYQNFVEKHGDLSAVPTRVFLAPPQIGEEVNVTIDQGKTLLIKLLAVGALDQQKGVRDVFFELNGEVRAVSILDNSAAVDHVQREKATREPGSVGAPMGGVIIELRVHEGQEVKEGDPLAVISAMKMENSISAPASGKIARVVVHESDSLSAGDLICEIAR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JH668226
EMBL· GenBank· DDBJ
EIM23063.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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