I3SAV1 · GLB32_LOTJA

Function

function

Hemoglobin-like protein that exhibits an unusual concentration-independent binding of O2 and CO (By similarity).
Required for general plant development and during nodulation (PubMed:34387337).
May promote shoot organogenesis from root explants (By similarity).

Features

Showing features for binding site.

116820406080100120140160
TypeIDPosition(s)Description
Binding site98Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); proximal binding residue

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionoxygen binding
Molecular Functionoxygen carrier activity
Biological Processnodulation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Group 2 truncated hemoglobin 3-2
  • Short names
    LjGlb3-2
  • Alternative names
    • Phytoglobin 3.2
      (Phytogb3.2
      )
    • Two-on-two hemoglobin 3-2
      (2-on-2 hemoglobin 3-2
      )

Gene names

    • Name
      GLB3-2
    • ORF names
      Lj1g3v0948590
    • Ordered locus names
      LotjaGi1g1v0172000

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > robinioid clade > Loteae > Lotus

Accessions

  • Primary accession
    I3SAV1

Phenotypes & Variants

Disruption phenotype

Reduced shoots, roots and leaves size and fewer leaves, but increased number of flowers and small pods containing less seeds (PubMed:34387337).
Delayed nodules formation of smaller size (PubMed:34387337).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004603041-168Group 2 truncated hemoglobin 3-2

Expression

Tissue specificity

Equally expressed in all organs, including root nodules, leaves, roots, stems, flowers and fruits.

Induction

Repressed by cytokinins (CK, an equimolar mixture of kinetin and 6-benzyl-aminopurine) in roots.

Developmental stage

In nodules, mainly localized in the cortex, and, to a lower extent, in the vascular bundles and infected tissues (PubMed:21073469).
As nodule aged, restricted in the mid-cortex and inner cortex, vascular bundles, and periphery of infected zone (PubMed:21073469).

Interaction

Subunit

Homodimer when ferric.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    168
  • Mass (Da)
    19,522
  • Last updated
    2012-09-05 v1
  • Checksum
    D68B22689B4EC341
MQSLQHKASEWSGVPSDEAFAIDDTNRFLKLGLQTFINLSTNFYNRVYDDDEEWFRSIFADSEKQNAIQNQYEFFVQRMGGPPLFSQRRGHPALIGRHRPFPVTHEAAERWLHHMQQALDSTPDIDDDSKIKMQNFFRHTAYFLVAGNELKKQNEQMPCKHAAGSDNS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BT137598
EMBL· GenBank· DDBJ
AFK37393.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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