I1TEM1 · T3O_CATRO
- ProteinTabersonine 3-oxygenase
- GeneCYP71D1V2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids506 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cytochrome P450 catalyzing the monooxygenation of 16-methoxytabersonine, 16-hydroxytabersonine and tabersonine, but not of 2,3-dihydrotabersonine (PubMed:25918424).
Converts the C2,C3 alkene of tabersonine and 16-methoxytabersonine to the epoxides, which then spontaneously open to form the corresponding imine alcohols (PubMed:25850027).
Inactive in converting amyrin to ursolic acid (PubMed:22837051).
Converts the C2,C3 alkene of tabersonine and 16-methoxytabersonine to the epoxides, which then spontaneously open to form the corresponding imine alcohols (PubMed:25850027).
Inactive in converting amyrin to ursolic acid (PubMed:22837051).
Miscellaneous
The CYP71D1V2 product is either trapped by the reductase T3R to form vindorosine and vindoline precursors, or it spontaneously rearranges to form a member of the eburnamine class of monoterpene indole alkaloids.
Catalytic activity
- 16-methoxytabersonine + O2 + reduced [NADPH--hemoprotein reductase] = (3R)-1,2-didehydro-3-hydroxy-16-methoxy-2,3-dihydrotabersonine + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.1 μM | tabersonine |
The KM is measured in the presence of a saturating amount of tabersonine 3-reductase, the enzyme converting the unstable intermediate produced by the reaction.
Pathway
Alkaloid biosynthesis; vindoline biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | NAD+ binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | indole alkaloid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTabersonine 3-oxygenase
- EC number
- Short namesT3O
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Apocynaceae > Rauvolfioideae > Vinceae > Catharanthinae > Catharanthus
Accessions
- Primary accessionI1TEM1
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Lumenal | ||||
Sequence: MEFHE | ||||||
Transmembrane | 6-26 | Helical | ||||
Sequence: SSPFVFITRGFIFIAISIAVL | ||||||
Topological domain | 27-506 | Cytoplasmic | ||||
Sequence: RRIISKKTKTLPPGPWKLPLIGNLHQFLGSVPYQILRDLAQKNGPLMHLQLGEVSAIVAASPQMAKEITKTLDLQFADRPVIQALRIVTYDYLDISFNAYGKYWRQLRKIFVQELLTSKRVRSFCSIREDEFSNLVKTINSANGKSINLSKLMTSCTNSIINKVAFGKVRYEREVFIDLINQILALAGGFKLVDLFPSYKILHVLEGTERKLWEIRGKIDKILDKVIDEHRENSSRTGKGNGCNGQEDIVDILLRIEEGGDLDLDIPFGNNNIKALLFDIIAGGTETSSTAVDWAMSEMMRNPHVMSKAQKEIREAFNGKEKIEENDIQNLKYLKLVIQETLRLHPPAPLLMRQCREKCEIGGYHIPVGTKAFINVWAIGRDPAYWPNPESFIPERFDDNTYEFTKSEHHAFEYLPFGAGRRMCPGISFGLANVELPLALLLYHFNWQLPDGSTTLDMTEATGLAARRKYDLQLIATSYA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000439949 | 1-506 | Tabersonine 3-oxygenase | |||
Sequence: MEFHESSPFVFITRGFIFIAISIAVLRRIISKKTKTLPPGPWKLPLIGNLHQFLGSVPYQILRDLAQKNGPLMHLQLGEVSAIVAASPQMAKEITKTLDLQFADRPVIQALRIVTYDYLDISFNAYGKYWRQLRKIFVQELLTSKRVRSFCSIREDEFSNLVKTINSANGKSINLSKLMTSCTNSIINKVAFGKVRYEREVFIDLINQILALAGGFKLVDLFPSYKILHVLEGTERKLWEIRGKIDKILDKVIDEHRENSSRTGKGNGCNGQEDIVDILLRIEEGGDLDLDIPFGNNNIKALLFDIIAGGTETSSTAVDWAMSEMMRNPHVMSKAQKEIREAFNGKEKIEENDIQNLKYLKLVIQETLRLHPPAPLLMRQCREKCEIGGYHIPVGTKAFINVWAIGRDPAYWPNPESFIPERFDDNTYEFTKSEHHAFEYLPFGAGRRMCPGISFGLANVELPLALLLYHFNWQLPDGSTTLDMTEATGLAARRKYDLQLIATSYA |
Expression
Tissue specificity
Expressed in leaf epidermis.
Induction
Up-regulated by herbivory.
Structure
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)57,443
- Last updated2012-07-11 v1
- Checksum2A19115E1F30B785
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JN613016 EMBL· GenBank· DDBJ | AEX07771.1 EMBL· GenBank· DDBJ | mRNA | ||
KP122967 EMBL· GenBank· DDBJ | AKM12282.1 EMBL· GenBank· DDBJ | mRNA | ||
LN831957 EMBL· GenBank· DDBJ | CQR79399.1 EMBL· GenBank· DDBJ | mRNA |