I1RY35 · ERG13_GIBZE
- ProteinHydroxymethylglutaryl-CoA synthase ERG13
- GeneRGE13
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids456 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Hydroxymethylglutaryl-CoA synthase; part of the first module of ergosterol biosynthesis pathway that includes the early steps of the pathway, conserved across all eukaryotes, and which results in the formation of mevalonate from acetyl-coenzyme A (acetyl-CoA) (By similarity).
ERG13 condenses acetyl-CoA with acetoacetyl-CoA to form hydroxymethylglutaryl-CoA (HMG-CoA) (By similarity).
The first module starts with the action of the cytosolic acetyl-CoA acetyltransferase ERG10B that catalyzes the formation of acetoacetyl-CoA. The hydroxymethylglutaryl-CoA synthases ERG13 then condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The rate-limiting step of the early module is the reduction to mevalonate by the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductases HMG1 (Probable)
ERG13 condenses acetyl-CoA with acetoacetyl-CoA to form hydroxymethylglutaryl-CoA (HMG-CoA) (By similarity).
The first module starts with the action of the cytosolic acetyl-CoA acetyltransferase ERG10B that catalyzes the formation of acetoacetyl-CoA. The hydroxymethylglutaryl-CoA synthases ERG13 then condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The rate-limiting step of the early module is the reduction to mevalonate by the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductases HMG1 (Probable)
Catalytic activity
- acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-hydroxy-3-methylglutaryl-CoA + CoA + H+This reaction proceeds in the forward direction.
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 86 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Active site | 118 | Acyl-thioester intermediate | ||||
Sequence: C | ||||||
Active site | 259 | Proton donor/acceptor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydroxymethylglutaryl-CoA synthase activity | |
Biological Process | acetyl-CoA metabolic process | |
Biological Process | farnesyl diphosphate biosynthetic process, mevalonate pathway | |
Biological Process | sterol biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHydroxymethylglutaryl-CoA synthase ERG13
- EC number
- Short namesHMG-CoA synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium
Accessions
- Primary accessionI1RY35
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454671 | 1-456 | Hydroxymethylglutaryl-CoA synthase ERG13 | |||
Sequence: MSSRPQNIGIKAIELYFPSQYVDQVELEKFDGVSAGKYTIGLGQTKMSFCDDREDIYSFALTATSKLLKNYNIDPNSIGFLEVGTETLLDKSKSVKSVLMQLFGDNTNIEGVDTINACYGGTNAVFNAINWVESSAWDGRDAIVVAGDIALYAKGNARPTGGAGAVALLIGPNAPIVAEPGLRGTYMQHAYDFYKPDLTSEYPYVDGHYSVNCYSKALDAAYRAYCKREAKQANGTNGVTNGDASTKTGLDRFDYMAFHSPTCKLVQKSYARLLYHDYLANADSPVFAEVAPELRDMDYEKSLTDKVVEKTFMTLTKKRFQERVNPAIQVATNCGNMYCGSVWSGLASLISVVDNKDLEGKRIGLFSYGSGLAASFLSFRINGSVDKISDVLNIPSRLESRRAVPPETYDQMCDLRKQAHLQKDYTPKGDPSTILPGTYYLTKVDDMFKREYAIKE |
Expression
Induction
Expression is regulated by the Zn2-C6 fungal-type transcription factor FgSR which binds directly to the promoter.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the thiolase-like superfamily. HMG-CoA synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length456
- Mass (Da)50,352
- Last updated2012-06-13 v1
- Checksum70DF1FAEFC94DE27
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HG970335 EMBL· GenBank· DDBJ | CEF82946.1 EMBL· GenBank· DDBJ | Genomic DNA |