I1RVD9 · FUSA2_GIBZE

Function

function

Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of the lipopeptide fusaristatin A (PubMed:25412204).
Fusaristatin A consists of a polyketide chain linked to three amino acid residues glutamine (Gln), dehydroalanine (dehydro-Ala), and beta-aminoisobutyric acid (PubMed:25412204).
The biosynthesis starts with formation of a linear polyketide chain by the highly reducing polyketide synthase PKS6 (PubMed:25412204).
The gene cluster does not contain an acyl-CoA ligase or an acyl-transferase, and it is therefore predicted that the polyketide is transferred directly to the nonribosomal peptide synthetase NRPS7 (Probable). Modules 1-3 from NRPS7 incorporate dehydro-Ala, Gln, and beta-aminoisobutyric acid in the compound, which is released by cyclization (PubMed:25412204).
The beta-aminoisobutyric acid units are most likely not freely available to the NRPS, but can be synthesized from thymine, which requires a dehydrogenase, a monooxygenase, and an aminotransferase. The fusaristatin A cluster contains a cytochrome P450 monooxygenase (FGSG_08207) and an aminotransferase (FGSG_17085), which theoretically can perform two of the enzymatic steps (Probable). The enzymes may however also be involved in biosynthesis of dehydroalanine or modification of the polyketide (Probable). The dehydro-Ala residue can be a result of cyclization, where serine is dehydrated (Probable). The last gene of the cluster encodes a protein with an A/B barrel domain found in variable enzymes, which hampers functional prediction (Probable)

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Molecular Functionisomerase activity
Molecular Functionligase activity
Molecular Functionphosphopantetheine binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nonribosomal peptide synthetase 7
  • EC number
  • Short names
    NRPS 7
  • Alternative names
    • Fusaristatin A biosynthesis cluster protein NRPS7

Gene names

    • Name
      NRPS7
    • ORF names
      FGRAMPH1_01T09373, FGSG_08209

Organism names

Accessions

  • Primary accession
    I1RVD9
  • Secondary accessions
    • A0A098DC07

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

impairs the production of fusaristatin A.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004453741-4423Nonribosomal peptide synthetase 7
Modified residue1570O-(pantetheine 4'-phosphoryl)serine
Modified residue2679O-(pantetheine 4'-phosphoryl)serine
Modified residue3765O-(pantetheine 4'-phosphoryl)serine

Keywords

Interaction

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

TypeIDPosition(s)Description
Region572-986Condensation 1
Region1007-1404Adenylation 1
Domain1533-1609Carrier 1
Region1657-2066Condensation 2
Region2102-2499Adenylation 2
Domain2642-2718Carrier 2
Region2764-3170Condensation 3
Region3205-3609Adenylation 3
Domain3731-3804Carrier 3
Region3875-4278Condensation 4
Region4288-4312Disordered
Compositional bias4298-4312Basic and acidic residues

Domain

NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D-amino acid conversion) are present within the NRP synthetase (By similarity).
NRPS7 has the following architecture: C-A-T-C-A-T-C-A-T-Cy. The last condensation domain (Cy) could be responsible for cyclization of the final product (Probable)

Sequence similarities

Belongs to the NRP synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    4,423
  • Mass (Da)
    488,395
  • Last updated
    2012-06-13 v1
  • Checksum
    E80EB1E8F4402D91

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias4298-4312Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HG970333
EMBL· GenBank· DDBJ
CEF76489.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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