I1RF60 · AURJ_GIBZE

Function

function

O-methyltransferase; part of the gene cluster that mediates the biosynthesis of aurofusarin, a red mycelium pigment which is acting as a mycotoxin (PubMed:15809006, PubMed:15811992, PubMed:16879655).
The first step is performed by the polyketide synthase which condenses one acetyl-CoA and 6 malonyl-CoA units to form the first intermediate, the cyclic heptaketide and yellow pigment YWA1 (PubMed:21296881, PubMed:23557488).
The C2 hydroxyl group in the pyrone ring of YWA1 is probably formed during ring closure by an aldol-type cyclization reaction (PubMed:21296881).
The dehydratase aurZ then acts as the first tailoring enzyme in the aurofusarin biosynthetic pathway by converting YWA1 to nor-rubrofusarin (PubMed:21296881, PubMed:23557488).
Nor-rubrofusarin is then methylated to rubrofusarin by the O-methyltransferase aurJ (PubMed:21296881, PubMed:23557488).
Rubrofusarin is then transported across the plasma membrane by the rubrofusarin-specific pump aurT for further enzymatic processing by the extracellular complex composed of GIP1, aurF, aurO and aurS to yield aurofusarin (PubMed:21296881).

Catalytic activity

Pathway

Pigment biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site283S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site338Proton acceptor

GO annotations

AspectTerm
Molecular FunctionO-methyltransferase activity
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    O-methyltransferase aurJ
  • EC number
  • Alternative names
    • Aurofusarin biosynthesis cluster protein J
    • Gibberella pigment protein 7

Gene names

    • Name
      aurJ
    • Synonyms
      GIP7
    • ORF names
      FG02326, FGRAMPH1_01T05597

Organism names

Accessions

  • Primary accession
    I1RF60

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004410881-439O-methyltransferase aurJ

Expression

Induction

Expression is regulated by the aurofusarin biosynthesis cluster-specific transcription factor aurR1/GIP2 (PubMed:16461721, PubMed:16879655).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    439
  • Mass (Da)
    48,089
  • Last updated
    2012-06-13 v1
  • Checksum
    F4C18EEAE425AE18
MGSISSPSLIIDLANAVSSAAKNLDTQLQSQGFPQPSFEADGPTYVVPKDAPKAAHEARVATAEAALKLFNLVSGPSELLPNMTASYHTIFALQWLHHFDVFSHIPLDGSLSYEKLATKANVPESLLKSVARMAMTSNILAEPTTGQVAHSANSAMFVKFPNMRDWASYMFTASIPTAAAMVQATEKWPGSVKKTETAYNIAFNHDLPFFDHLSQSPVMTKQFSGYMRSVTDGQGMDLSHLVNGFDWASLPDKSLIVDIGGSAGHASYALAAAYPHLRFEVQDLDTVVNGEKAAKEHEEAVSKHVIGTDNRVTFKAHNFFEAQPTKDATVYMLRMIIHDWPDAEAKTILGNLVPALESAKATLLIMDTVLPSPGSIPSVRERVIRTRDLTMRQVFNAKERGVDDWEAILRETDSRLTLKNLRQPEGSNMCLLTISLQDD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HG970332
EMBL· GenBank· DDBJ
CEF74603.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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