I1RF49 · NRPS4_GIBZE
- ProteinNonribosomal peptide synthetase 4
- GeneNRPS4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids7639 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of the fusahexin, a cyclic hydrophobic hexapeptide with the amino acid sequence cyclo-(D-Ala-L-Leu-D-allo-Thr-L-Pro-D-Leu-L-Leu) that plays an important role in cell surface hydrophobicity (PubMed:22862913, PubMed:34292732).
Fusahexin might also play a role in virulence, sensitivity to osmotic stress and oxidative stress (PubMed:34292732).
NRPS4 is the only enzyme within the cluster and its 5 catalytic modules are sufficient to produce fusahexin (PubMed:34292732).
The modules 1 to 4 incorporate respectively D-alanine, L-leucine, D-allo-threonine, and L-proline, which is supported by the presence of epimerase domains in modules 1 and 3, which incorporate D-amino acids (PubMed:34292732).
The terminal module is responsible for incorporation of the two adjacent leucine units, where the epimerase domain is only used to convert the first unit to D-leucine (PubMed:34292732).
The terminal condensation domain (Ct) is involved in cyclization with D-alanine and thereby releasing of fusahexin (PubMed:34292732).
Fusahexin might also play a role in virulence, sensitivity to osmotic stress and oxidative stress (PubMed:34292732).
NRPS4 is the only enzyme within the cluster and its 5 catalytic modules are sufficient to produce fusahexin (PubMed:34292732).
The modules 1 to 4 incorporate respectively D-alanine, L-leucine, D-allo-threonine, and L-proline, which is supported by the presence of epimerase domains in modules 1 and 3, which incorporate D-amino acids (PubMed:34292732).
The terminal module is responsible for incorporation of the two adjacent leucine units, where the epimerase domain is only used to convert the first unit to D-leucine (PubMed:34292732).
The terminal condensation domain (Ct) is involved in cyclization with D-alanine and thereby releasing of fusahexin (PubMed:34292732).
Catalytic activity
- A + D-alanine + D-allo-threonine + D-leucine + 2 L-leucine + L-proline = AH2 + fusahexin + 6 H2OThis reaction proceeds in the forward direction.
Pathway
Secondary metabolite biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | isomerase activity | |
Molecular Function | ligase activity | |
Molecular Function | phosphopantetheine binding |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNonribosomal peptide synthetase 4
- EC number
- Short namesNRPS 4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium
Accessions
- Primary accessionI1RF49
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Does not affect conidiation but results in reduction of cell surface hydrophobicity.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455674 | 1-7639 | Nonribosomal peptide synthetase 4 | |||
Sequence: MAPATCCCCADSASQAMPDMEPTIFPASEIKEEIQTMISSYSSRLGHDFNQDLAKPCNMFCLAWAILLERFTGLDNVCFGFRSEHMVTAGSERTGGVASAMQVQVTADHSVQENLDENAFVSVMIPETKAETLFNTTLSICNLGNQPLCEDEARKIDVLCKLHMSVDPATMKTLLSWDSTFMTREQAETISNTYDKIFRELSSKKRTTVSAIHCCSEQDELKILSWNGSPLNNVQKCIHQAVSWQGAMRPDAEAVCSWDGSFSYAQLLSLSDRLAFHLKKLGVGAETFVPICFDKSKWTIVAMLAILKAGGIFVPLDPTQPLLRLQNLTRKVDADTILCSPQHQEMIESIASKVIPVDAQLFESLAEQRGEVDCGSWSSGAYMIFTSGTTGEPKGALIQHGALLSSALAHGPAMMMDNNTRSLHFAASTFDVSITEILTCLILGGCVCIPSEEARLNAIEEAITQLRVNWALLTPTFVKFINPDNVPSLKTLVTGGEAMTQAVIRSWSHINLINCYGPAETSVVSHVHRGMREGKNPLNIGHQVGIHCWVVDRYNHNRLMPVGAVGELVIESHTLAREYYKEPEKTSEAFIVDPEWALNQPHHSSPRRMYKTGDLVRYNYDSSFHIAGRKDAQIKFHGQRIELGEIEYHINVGINIKHGMVVLPKAGFCEGRLLAIVQLSDASGHDLVPNGRPYQLIDGPLEQVAIAKVEETKQLLTERLPSYMVPSMWLAVEFIPRLQSGKLDRKQTGKWVEDMTEETYRKLNPVAVGDPSESLTFSNGTESQLHNIWTHVLNLKPEQLGLSQSFLSVGGDSISAMQVMSECKKRGLGLTVSHIISCKSISALARHVKEIEKPMLLQETTETPFELSPIQRLYFSRSNHDQGHYNQSFLLRVSRRIDESAMRRAIEVVIRKHSMLRARFSRDDTGRWQQRVTDKVESSYRLRSIQLSSNEELSHALIDSQTCLDYANGPLLAADLLDEEGQDQRLFVVAHHLVIDLVSWRIVLQELEELLLRPELKPDMDRPLPFQTWCQMQREHASAQTPEQALPIHGIPDGDPTYWGMEDTPNIYGQMVHEGFEVGSAQTSLLLSKCHDALRTEIPDVLMAAMVYSFGQTFTDRQTPAIFAEGHGRESWDPSIDLSNVVGWFTTIYPVFADSDATSTLIDTIKMVKDGRRKVPDNGRPYFASRWLTESGEKAFARHWPLEITFNYLGQYQQLEREGALFIPVKGIAGEVSSATDGADVGSLATCISLFEVSAVITKGTLRFSFLFNQNMKHQPKIRQWIASCEQNLSLLVESLAVMSPEPTLSDFPLVSLTYDRLRLLTQEKLPEVGIEDMGRVEDIYPCSPMQSGLLVSTTKDSAAYAAYTLHQVKSRSGGAVDVTKLADAWKRMVDYHPMLRTVFVESVTLDESLFDQVLLKEVKVPLVMSELGTDEEAIKTLDKARHHDEYSQLLHVFEICKTTSGNVFCKLDISHVIMDGTSLSILFRDLSLAYAGILGPDKGPPYSEYIRSLQHQGLQHGIEYWKSYLMGIEPCHFPVLDDGEVVDTRESKCFRVEFDELAQLQRLCDDRGVTIVNAIYAAWALVLRLYTASEEVCFGYLTSARDSQIEGIEDVVGPVINMVTCRANISDSTTLGDTMTVVQNDFLNSLKHRHIPLAQVQHALKLSDVALFNTALSYRKLPPALQDAPDVMFEEVRPTYDPDEYDVSINIEAGENDMMIDLTYWSDTMSDGQATNVASAFTTALSNILHHSDKPVAQLNHLGPRHHQQISQWNNVIPEAVESCVHGLFEEQAILRPEAPAITSWDADFTYAELDTTSTKLAHYLADLGVGLEQFVLVCFDKSAFAIVAMLAVLKAGGVCVPLDPAHPDAAIRLRAEDTGASIAAVSSSMASRLSNIVDKAVVVDSNLLQNISENAILPQINPHNACFVIYTSGSTGRPKGVVLEHRGIATNAKSSGPKLGYSEESRVLQFASYTFDNSLAEIFTTLALGGCVCVPSEHERFNDLAGAISRYRVTLADITPTVACLINPLDVPTLKTLALGGEAVTHKCVDIWRDFVSLQCCYGPSECSVNSTYSGEIAQPGKATNIGRAVGSVTWVVDATDHNRLVPIGCIGELLIDGPIVSRGYLNLPEKMAQSFVAPPASLGDMCREGNLSRKLYKTGDLVRYNSDGTLTYFGRKDTQVKLHGQRIELEEIEHHLEQNLPQDWTSAVELIQFEGKKSLASFICADLGSVRSASNEKNTVLAMDDSFRSLAKELEIALSNNLPAYMIPSVWLPVSEMPMTSSGKLDRRSLRSMVQSLPASEMTSYKLALKSGRAPASDMEKQLASMWAHVLNVDANTIGVEDHFFRLGGDSIAAMQLVTLARKSNINLTVTGVFQKGSLLDMAQSALPLSRTAVATVYAFSLLPEVVSLDALKEEIGSCARIQVGDVEDIYPCTPLQQGLMALSAKEPGAYVAQLVFRLPNGTDLDNFKMAWRLVIEAEGTLRTRLVQTTDHGILNVVVKGDVPQWSTDRSLSDLQRLRSHLPSSNGGRLTDYAIVEDGSDVSFVWTIHHALYDGWCLPLILDRVKQCYEGIQSSTSEPIASGPTYSRFIRYLNETDSSQDVKFWESYLSDISTQHFPRLPDPDYKPSASGLIIHKTCFDNTRDGMKSVGLGITTATMIRSAWALTVSTYAASDDVVFWETMTGRDVPVEGIEEMVGATLSTVPTRIALDRSQKVSDLLSSVQAQSAVVRMHQFAGIHTIKRINTDTAFACGAQNLLAINYGPRTSTDSFWCDQTNEMAGTNFYSYPLMLSCHVADGELETVVHFDPDVICESQMHRVMDQFALMLTAVTSKDLVDEKLSELDLISTRDYQSLSEMNGQMVPSCDMCVHDVIKASGIAQPLDKLAVCAWDQNLTYEGLDSQSTHLSSALIEAGVRPNTFVPFCMEKSSMVVVSILAILKSGAAFVPLDYAHPDARISGIIADVEAEFVLSSPQYAERLTKLGAKVISVSKDTIQDSMPLQQHDLSVSTKSPAYCIFTSGTTGRPKGTIIDHSAFCTGALAHGKAMGMNESSRVLQFASHTFDASIMETLSTLIHGGTVCVPSEEERSQDIAGFIRRMSVNWALLTPSVAQLIEPSTVPELKTLVLGGEAMSRAHLSTWAPSVQLMNAYGPSETSVVATVNSNVTLDSSPANIGRAVGGLCWVVDSANADRLLPIGVVGELFVEGPILSQGYLKNSQKNAESFITNPRWCSKFTSETASSERRFYRTGDLVKITEDGSIEFQGRKDNQVKINGQRLELSEIEHHLNTDAIVQACLAFIPTTGPLKSRLVAVLSLHSTFVSRGPDEMQLVIDFARSELTSVRDSLTGQLASYMIPSMWIVVNRIPLLPSGKLDRRRVANWVEAMSPEQYQLAIGAQDESYASGLDREATETETKLRAAWAKVLGIEVESVPFTRSFIQLGGDSISAMQLVAICRSSNMALSVSQIMQSKSIVKLASFVQAVEDVTQDDEQEDKAFPLSPIQKLYFERMYSDSTHFNQSMVLETTRKITPQDLSNALEAIVKTHSMLRARFSDVDGAYSQRITSDIAGSYAFESHVGMDQCRVSELIEKTQKSLNIFQGPLLAAASIEMEDIDKQIVFLAVHHLVVDVVSWNIILQDLEGLLSSSASNLGKPLSFQTWNTLQLEESRNQTPDRVFHNLPTPAQDLAYWGMQAVPNIHGDAIAETIEIASDVSLQLLGPCHEALNTNVVDVLLASLLSSFHQAFPDRLSMPTIFNEGHGREPADNKLDLSRTVGWFTTLCPVYLPDSLPVQPDILDIICWVRDFRRRIPGNGRPYFAHQMLSESGQKESAEWPAELAFNYLGQRQKTELEGSLFKSPDGVLASVGSETDIGADVPRLALIEISASFSRDDLSFSFSYSRQMKHQPCIREWVKNFSATLQTAVERMTQAKAEPQDLDTSLLPLAFRATSKVDTRLAELGISCCADVEAVYPCSPVQMGILFAQIRNPEFYSYSVTFGVNCIEPSRVVDVQRLKDAWQRVVQRHSTLRTTFVDGLLEEGGINQVVLRNHCADISVFERADNDELQMITERIKPKIMSNKPPHHLSIFSSAEGKVTCVLEMSHALSDGTSMPILFRDLAMAYEGSLDPTIVSAYRDYVSYLQCQGPNDIEYWREYLTGAEPCHLPLASKSTLPRALGYLDQTISCAADLQAFCTGAGVTLSNVIQLAWALVLQAYTGHDDVCFGYLLADRDAPVDNIDNAIGVFINMLVLRVRLGSSQSVGDALGAVQQDLSAAIGHKNISLTDIQRVTGLLNEPLFNTAYSFQRRSISKSMANGSLSFDVREAQDPNEYDLTVNVEVWDQAAELQLCYWTDKISNSQAKTIASTFDKILTSIATCDLSLPTNQLDIVSDDCAQQLTRWNNTEPTLLDQCVHHVFERNVQSLPHDTPAIEAWDARFTYSEVDMLSSRLAHHLVSLGILPEMYVPLCFEKSAWTPIAMLAVLKAGAAFVPIDPTHPPERIEFLVQNTSAKLILCSTSLAEKFDIGVPFLAIDHETMSTLSALPVTSPSIAVQPNNAAYIIFTSGTTGLPKGTIVEHAAFTTGGTAHAAAIKMTCSSRVLQFASYTFDASIMEILTTFLVGGCVCVPSDEERMNDLAGTMAKYDVNWALLTPSVAKVLKPGSVPGLKVLVTGGEAMSTDHITKWLGHAALINAYGPSEASVIAASHTKVDENGVILNEEPANIGHAVGCRTWVVDPHNHNHLMPIGSIGELLLEGPILARGYLKNETKTTDAFIDYPPWRANMSLSGDRVDRMYKTGDLVSQNSDGSLNYVSRKDTQIKLNGQRIELGEIEHHVRANLPAHVQSAVELVVPQSKTSTKTLAAFFTVDDHEVLKETSDPLLPMSSAYMEIGQSLKTALRVALPTHMVPTMYVPLTKMPWTSAGKLDRQKLKTIVQSIAPQDIGGYKLVGASNSRAPTTMMQRKLQKIWAGILNIHPSTISIDDSFFRLGGDSISAMKVVSAARMEEISLTVMDILTSPTLSEMATCCGHSENTTVMEVEPFSLLHDVDSPPSLLDEVADCCDVPTSQIQDLYPCSSLQEGLVAASMQQPGAYVARYIFKVPSTIDMERLKMAWQNTSNHVDILRSRIVNARSLKTYQAILEPHAINWEHYTSLEAIADKTIQLPERNGGVLAKYAIIDSSDPDLRYFVWSVHHALYDAWSMPSLLNLVSQFYHEATTEQLAPPVPYANFARYLVDSDAQASDEFWKATFQNASGVSHFPTATLSDEESTYSSLQHTIQCRRDDLGADITIPTIVRAAWALLLGAHTGSDDVGFGETLSGRDIALEHVEDILGPTLTTVPWRVQIDRSATVGHFLHSLHKKSAEVIPHQHAGLQHIKRLGGSIAVASDFRNLLVIQASDEATDHQDLLQPLEENGNHKNFFTYPLVVECSIELNNLVLTIHHNETVMTSWQVERLAHQFDALVNQLSRLSQEPDRKVAELQFCSEEDLQMIKGWNNGTCDAVYDTIPSLFWQSVATYHDATSIRAWDGHLTYGSLAQHAGHLAKRLIQEGVKAETMVPCCMDKSLWTTVAMLAVVLAGGTIIPMDPAHPRARHAEIARECKAIIALCSPEYRDRFIGVVPTVIAIDQTLFTKQLCQDHIASEDLPLVADKDAAFVIYTSGSTGKAKGVVIEHGSFVASSRAYIKHMNLSATSSVFHFTSYAFDIAMGETFGALTTGACLCVPSEEMRVTDLPGVMNTLGATWAFLTPSLANMQDPSMFKTLQALVCGGEAMTSETVSKWSNKVKLINGYGPAECTVFALSNSNVSEDQDHSNIGRAMDGCQTWIVDTRDHNKLVPVGCEGELLISGPILSRGYLNDSAKTSKSFIENPAWMHHFDDKKHQNPVRLYKTGDLVRYRPDGNLTFIGRKDNQVKLHGQRMELGEIEACLESDPRLRNALVALPKSGVFKGRLVAVLSFKDSDSHNPGLVSSQFSPISESDMDVARLHLPDLQQILSENLPPYMMPSSWLVVEAIPLLLSGKLDRASTQKWLTEMVAETPEFLLDQQLGQDAGATDTTFVGQLLRKIWASVLRIPDESKLSGRSFISLGGDSIMAMQVMSRCRDHSIQLTMRDIMSGKSISDLVTLIEKEGRGKQTVNPEYEEDNSRPFALSPIQQLFFNNSNDKDRGDRFNQSQLFSITESIDKDTFANAIHALVQRHPMLRARFNKSSTTGQWSQQVAPDTEDSYGLHFHEVSDASQIARQIAASQESIGISGPVFIVDLFKMPDGHHHVSMIAHHLVVDVVSWINIAQDLETLLSTSPSMSSKPLSFRKWNAAQTEHATSVAKKNEDLLPFTVRPANLDYWGVSGVSNSYSQVTQQSFSLSDVDTVSLLLGDAHQALRTEPLDLFISALLISFGQCFPDRELPTLFNEGHGREPWDDSIDLSQTVGWFTSLCPIDISHHNADPTYTIDYVRKVKDVRRAIPGNGRPYFAQRYLTDSGKQSLDAHEPMEILLNFLGRSQQSGEDDSILRLSNLSMSDEDMASMSDVGPETRRLALFELSISILDEGIQFTFMYNKNMLHQDLIQQWVITCKEVLGNMAMELSTAPSCPTISDFPLMSLDYAELNKLVTKSLPTAHVRFDEVEDMYPCSPMQMGILISQLLDPSQYLFYAVLEVSASSRSAIDSAKLAQACSEVVERHDALRTVFIESVRSGGSFDQVVLRPGKPRIATFKCREIDVMAKLNTRSLGKTNKRHGVPILPYQITICETPQGKVFIKLEMNHAVTDGASTAIVLRDISSAYANNLHPTKAPSYKEYINYITKQPSDSSLMYWKSYLYGARNTEFPAMNSDHISGRSLGSIAVDFDRFSELQSLGLDAGVTFSNMIMVAWALVLRKYTNSQDVCFGYLGSGRDADIDGVDEIVGPLINMLVFRFQFTHSMLLKRLFLDTQEDYANSLPHQHFSLARVSHELGQSKRGFFNTAVSIQNAGASSDADFSALKFESVDAFDPSEYAVTLNANTTRGDEGIVFRYWTNILSHSQAKELAIVMSEVLSDMIDHSEEALSHLRVSQDSSLPTNPAQDLHGWTFEHSDTSEQFKTTNSTISSYSTGPGATLFSPATSWGSLPRDKDQVYNKLSALWKQHLDVATTDLTYDGSFFEYGGDSIIAMAMVGDARDRDLPLTVADIFKNPSFGTLLNCLRDKSYREGDMVSSDGNISLSGSKKEGIVVDEHTYEPLSLLPQQNAEQFVREHVCPVVGVSRASITDVLPTTDFQAQAIEGSLLDSRWMLNYFHLDGEGPLDVALLQESITNVIASYDVLRTVFVPYEATYLQVILRHVQSELIFHDVDDVEQFTLDLESDHLRQIPSPEKPSLRFILARHEPSERHRLFIRLSHALYDGVCFPAILNALKASYEGEPIATTPSYATYIHGLFSKANPDQHTYWRSLLEGSAPTNLIPRECRSMRTNPTQALRKIVATPSLATVNITTATVIKAAWSVVLAKNTGTRDVVFGHLISGRNSCHVPGIEAIVGPCLNVVPVRVQYQDSWMVLDLLQHIQHQQVDNIPHESLGFREIIRNCTNWDDDGANGFSTVVQHQSMAQTGSLDIGDNTYEVGVIASQEDTADFSVVTTPQDSSNTEVCFLYREGGVERTEFAEKLFDCLCSTIGDLSRDVKTPLVSWL | ||||||
Modified residue | 813 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 2350 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 3440 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 4981 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 6076 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 7125 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 244-636 | Adenylation 1 | ||||
Sequence: WQGAMRPDAEAVCSWDGSFSYAQLLSLSDRLAFHLKKLGVGAETFVPICFDKSKWTIVAMLAILKAGGIFVPLDPTQPLLRLQNLTRKVDADTILCSPQHQEMIESIASKVIPVDAQLFESLAEQRGEVDCGSWSSGAYMIFTSGTTGEPKGALIQHGALLSSALAHGPAMMMDNNTRSLHFAASTFDVSITEILTCLILGGCVCIPSEEARLNAIEEAITQLRVNWALLTPTFVKFINPDNVPSLKTLVTGGEAMTQAVIRSWSHINLINCYGPAETSVVSHVHRGMREGKNPLNIGHQVGIHCWVVDRYNHNRLMPVGAVGELVIESHTLAREYYKEPEKTSEAFIVDPEWALNQPHHSSPRRMYKTGDLVRYNYDSSFHIAGRKDAQIKF | ||||||
Domain | 776-852 | Carrier 1 | ||||
Sequence: TFSNGTESQLHNIWTHVLNLKPEQLGLSQSFLSVGGDSISAMQVMSECKKRGLGLTVSHIISCKSISALARHVKEIE | ||||||
Region | 865-1295 | Epimerization 1 | ||||
Sequence: FELSPIQRLYFSRSNHDQGHYNQSFLLRVSRRIDESAMRRAIEVVIRKHSMLRARFSRDDTGRWQQRVTDKVESSYRLRSIQLSSNEELSHALIDSQTCLDYANGPLLAADLLDEEGQDQRLFVVAHHLVIDLVSWRIVLQELEELLLRPELKPDMDRPLPFQTWCQMQREHASAQTPEQALPIHGIPDGDPTYWGMEDTPNIYGQMVHEGFEVGSAQTSLLLSKCHDALRTEIPDVLMAAMVYSFGQTFTDRQTPAIFAEGHGRESWDPSIDLSNVVGWFTTIYPVFADSDATSTLIDTIKMVKDGRRKVPDNGRPYFASRWLTESGEKAFARHWPLEITFNYLGQYQQLEREGALFIPVKGIAGEVSSATDGADVGSLATCISLFEVSAVITKGTLRFSFLFNQNMKHQPKIRQWIASCEQNLSLLVES | ||||||
Region | 1337-1767 | Condensation 1 | ||||
Sequence: VEDIYPCSPMQSGLLVSTTKDSAAYAAYTLHQVKSRSGGAVDVTKLADAWKRMVDYHPMLRTVFVESVTLDESLFDQVLLKEVKVPLVMSELGTDEEAIKTLDKARHHDEYSQLLHVFEICKTTSGNVFCKLDISHVIMDGTSLSILFRDLSLAYAGILGPDKGPPYSEYIRSLQHQGLQHGIEYWKSYLMGIEPCHFPVLDDGEVVDTRESKCFRVEFDELAQLQRLCDDRGVTIVNAIYAAWALVLRLYTASEEVCFGYLTSARDSQIEGIEDVVGPVINMVTCRANISDSTTLGDTMTVVQNDFLNSLKHRHIPLAQVQHALKLSDVALFNTALSYRKLPPALQDAPDVMFEEVRPTYDPDEYDVSINIEAGENDMMIDLTYWSDTMSDGQATNVASAFTTALSNILHHSDKPVAQLNHLGPRHHQQI | ||||||
Region | 1786-2181 | Adenylation 2 | ||||
Sequence: FEEQAILRPEAPAITSWDADFTYAELDTTSTKLAHYLADLGVGLEQFVLVCFDKSAFAIVAMLAVLKAGGVCVPLDPAHPDAAIRLRAEDTGASIAAVSSSMASRLSNIVDKAVVVDSNLLQNISENAILPQINPHNACFVIYTSGSTGRPKGVVLEHRGIATNAKSSGPKLGYSEESRVLQFASYTFDNSLAEIFTTLALGGCVCVPSEHERFNDLAGAISRYRVTLADITPTVACLINPLDVPTLKTLALGGEAVTHKCVDIWRDFVSLQCCYGPSECSVNSTYSGEIAQPGKATNIGRAVGSVTWVVDATDHNRLVPIGCIGELLIDGPIVSRGYLNLPEKMAQSFVAPPASLGDMCREGNLSRKLYKTGDLVRYNSDGTLTYFGRKDTQVKL | ||||||
Domain | 2313-2389 | Carrier 2 | ||||
Sequence: APASDMEKQLASMWAHVLNVDANTIGVEDHFFRLGGDSIAAMQLVTLARKSNINLTVTGVFQKGSLLDMAQSALPLS | ||||||
Region | 2426-2852 | Condensation 2 | ||||
Sequence: VEDIYPCTPLQQGLMALSAKEPGAYVAQLVFRLPNGTDLDNFKMAWRLVIEAEGTLRTRLVQTTDHGILNVVVKGDVPQWSTDRSLSDLQRLRSHLPSSNGGRLTDYAIVEDGSDVSFVWTIHHALYDGWCLPLILDRVKQCYEGIQSSTSEPIASGPTYSRFIRYLNETDSSQDVKFWESYLSDISTQHFPRLPDPDYKPSASGLIIHKTCFDNTRDGMKSVGLGITTATMIRSAWALTVSTYAASDDVVFWETMTGRDVPVEGIEEMVGATLSTVPTRIALDRSQKVSDLLSSVQAQSAVVRMHQFAGIHTIKRINTDTAFACGAQNLLAINYGPRTSTDSFWCDQTNEMAGTNFYSYPLMLSCHVADGELETVVHFDPDVICESQMHRVMDQFALMLTAVTSKDLVDEKLSELDLISTRDYQSL | ||||||
Region | 2878-3269 | Adenylation 3 | ||||
Sequence: QPLDKLAVCAWDQNLTYEGLDSQSTHLSSALIEAGVRPNTFVPFCMEKSSMVVVSILAILKSGAAFVPLDYAHPDARISGIIADVEAEFVLSSPQYAERLTKLGAKVISVSKDTIQDSMPLQQHDLSVSTKSPAYCIFTSGTTGRPKGTIIDHSAFCTGALAHGKAMGMNESSRVLQFASHTFDASIMETLSTLIHGGTVCVPSEEERSQDIAGFIRRMSVNWALLTPSVAQLIEPSTVPELKTLVLGGEAMSRAHLSTWAPSVQLMNAYGPSETSVVATVNSNVTLDSSPANIGRAVGGLCWVVDSANADRLLPIGVVGELFVEGPILSQGYLKNSQKNAESFITNPRWCSKFTSETASSERRFYRTGDLVKITEDGSIEFQGRKDNQVKI | ||||||
Domain | 3403-3479 | Carrier 3 | ||||
Sequence: REATETETKLRAAWAKVLGIEVESVPFTRSFIQLGGDSISAMQLVAICRSSNMALSVSQIMQSKSIVKLASFVQAVE | ||||||
Region | 3491-3928 | Epimerization 2 | ||||
Sequence: AFPLSPIQKLYFERMYSDSTHFNQSMVLETTRKITPQDLSNALEAIVKTHSMLRARFSDVDGAYSQRITSDIAGSYAFESHVGMDQCRVSELIEKTQKSLNIFQGPLLAAASIEMEDIDKQIVFLAVHHLVVDVVSWNIILQDLEGLLSSSASNLGKPLSFQTWNTLQLEESRNQTPDRVFHNLPTPAQDLAYWGMQAVPNIHGDAIAETIEIASDVSLQLLGPCHEALNTNVVDVLLASLLSSFHQAFPDRLSMPTIFNEGHGREPADNKLDLSRTVGWFTTLCPVYLPDSLPVQPDILDIICWVRDFRRRIPGNGRPYFAHQMLSESGQKESAEWPAELAFNYLGQRQKTELEGSLFKSPDGVLASVGSETDIGADVPRLALIEISASFSRDDLSFSFSYSRQMKHQPCIREWVKNFSATLQTAVERMTQAKAEPQ | ||||||
Region | 3961-4389 | Condensation 3 | ||||
Sequence: EAVYPCSPVQMGILFAQIRNPEFYSYSVTFGVNCIEPSRVVDVQRLKDAWQRVVQRHSTLRTTFVDGLLEEGGINQVVLRNHCADISVFERADNDELQMITERIKPKIMSNKPPHHLSIFSSAEGKVTCVLEMSHALSDGTSMPILFRDLAMAYEGSLDPTIVSAYRDYVSYLQCQGPNDIEYWREYLTGAEPCHLPLASKSTLPRALGYLDQTISCAADLQAFCTGAGVTLSNVIQLAWALVLQAYTGHDDVCFGYLLADRDAPVDNIDNAIGVFINMLVLRVRLGSSQSVGDALGAVQQDLSAAIGHKNISLTDIQRVTGLLNEPLFNTAYSFQRRSISKSMANGSLSFDVREAQDPNEYDLTVNVEVWDQAAELQLCYWTDKISNSQAKTIASTFDKILTSIATCDLSLPTNQLDIVSDDCAQQLT | ||||||
Region | 4407-4810 | Adenylation 4 | ||||
Sequence: FERNVQSLPHDTPAIEAWDARFTYSEVDMLSSRLAHHLVSLGILPEMYVPLCFEKSAWTPIAMLAVLKAGAAFVPIDPTHPPERIEFLVQNTSAKLILCSTSLAEKFDIGVPFLAIDHETMSTLSALPVTSPSIAVQPNNAAYIIFTSGTTGLPKGTIVEHAAFTTGGTAHAAAIKMTCSSRVLQFASYTFDASIMEILTTFLVGGCVCVPSDEERMNDLAGTMAKYDVNWALLTPSVAKVLKPGSVPGLKVLVTGGEAMSTDHITKWLGHAALINAYGPSEASVIAASHTKVDENGVILNEEPANIGHAVGCRTWVVDPHNHNHLMPIGSIGELLLEGPILARGYLKNETKTTDAFIDYPPWRANMSLSGDRVDRMYKTGDLVSQNSDGSLNYVSRKDTQIKL | ||||||
Domain | 4944-5020 | Carrier 4 | ||||
Sequence: APTTMMQRKLQKIWAGILNIHPSTISIDDSFFRLGGDSISAMKVVSAARMEEISLTVMDILTSPTLSEMATCCGHSE | ||||||
Region | 5058-5478 | Condensation 4 | ||||
Sequence: QDLYPCSSLQEGLVAASMQQPGAYVARYIFKVPSTIDMERLKMAWQNTSNHVDILRSRIVNARSLKTYQAILEPHAINWEHYTSLEAIADKTIQLPERNGGVLAKYAIIDSSDPDLRYFVWSVHHALYDAWSMPSLLNLVSQFYHEATTEQLAPPVPYANFARYLVDSDAQASDEFWKATFQNASGVSHFPTATLSDEESTYSSLQHTIQCRRDDLGADITIPTIVRAAWALLLGAHTGSDDVGFGETLSGRDIALEHVEDILGPTLTTVPWRVQIDRSATVGHFLHSLHKKSAEVIPHQHAGLQHIKRLGGSIAVASDFRNLLVIQASDEATDHQDLLQPLEENGNHKNFFTYPLVVECSIELNNLVLTIHHNETVMTSWQVERLAHQFDALVNQLSRLSQEPDRKVAELQFCSEEDLQM | ||||||
Region | 5498-5900 | Adenylation 5 | ||||
Sequence: FWQSVATYHDATSIRAWDGHLTYGSLAQHAGHLAKRLIQEGVKAETMVPCCMDKSLWTTVAMLAVVLAGGTIIPMDPAHPRARHAEIARECKAIIALCSPEYRDRFIGVVPTVIAIDQTLFTKQLCQDHIASEDLPLVADKDAAFVIYTSGSTGKAKGVVIEHGSFVASSRAYIKHMNLSATSSVFHFTSYAFDIAMGETFGALTTGACLCVPSEEMRVTDLPGVMNTLGATWAFLTPSLANMQDPSMFKTLQALVCGGEAMTSETVSKWSNKVKLINGYGPAECTVFALSNSNVSEDQDHSNIGRAMDGCQTWIVDTRDHNKLVPVGCEGELLISGPILSRGYLNDSAKTSKSFIENPAWMHHFDDKKHQNPVRLYKTGDLVRYRPDGNLTFIGRKDNQVKL | ||||||
Domain | 6039-6115 | Carrier 5 | ||||
Sequence: TDTTFVGQLLRKIWASVLRIPDESKLSGRSFISLGGDSIMAMQVMSRCRDHSIQLTMRDIMSGKSISDLVTLIEKEG | ||||||
Region | 6133-6567 | Epimerization 3 | ||||
Sequence: FALSPIQQLFFNNSNDKDRGDRFNQSQLFSITESIDKDTFANAIHALVQRHPMLRARFNKSSTTGQWSQQVAPDTEDSYGLHFHEVSDASQIARQIAASQESIGISGPVFIVDLFKMPDGHHHVSMIAHHLVVDVVSWINIAQDLETLLSTSPSMSSKPLSFRKWNAAQTEHATSVAKKNEDLLPFTVRPANLDYWGVSGVSNSYSQVTQQSFSLSDVDTVSLLLGDAHQALRTEPLDLFISALLISFGQCFPDRELPTLFNEGHGREPWDDSIDLSQTVGWFTSLCPIDISHHNADPTYTIDYVRKVKDVRRAIPGNGRPYFAQRYLTDSGKQSLDAHEPMEILLNFLGRSQQSGEDDSILRLSNLSMSDEDMASMSDVGPETRRLALFELSISILDEGIQFTFMYNKNMLHQDLIQQWVITCKEVLGNMAMEL | ||||||
Region | 6607-7032 | Condensation 5 | ||||
Sequence: VEDMYPCSPMQMGILISQLLDPSQYLFYAVLEVSASSRSAIDSAKLAQACSEVVERHDALRTVFIESVRSGGSFDQVVLRPGKPRIATFKCREIDVMAKLNTRSLGKTNKRHGVPILPYQITICETPQGKVFIKLEMNHAVTDGASTAIVLRDISSAYANNLHPTKAPSYKEYINYITKQPSDSSLMYWKSYLYGARNTEFPAMNSDHISGRSLGSIAVDFDRFSELQSLGLDAGVTFSNMIMVAWALVLRKYTNSQDVCFGYLGSGRDADIDGVDEIVGPLINMLVFRFQFTHSMLLKRLFLDTQEDYANSLPHQHFSLARVSHELGQSKRGFFNTAVSIQNAGASSDADFSALKFESVDAFDPSEYAVTLNANTTRGDEGIVFRYWTNILSHSQAKELAIVMSEVLSDMIDHSEEALSHLRVSQ | ||||||
Domain | 7088-7164 | Carrier 6 | ||||
Sequence: RDKDQVYNKLSALWKQHLDVATTDLTYDGSFFEYGGDSIIAMAMVGDARDRDLPLTVADIFKNPSFGTLLNCLRDKS | ||||||
Region | 7254-7603 | Condensation 6 | ||||
Sequence: LDGEGPLDVALLQESITNVIASYDVLRTVFVPYEATYLQVILRHVQSELIFHDVDDVEQFTLDLESDHLRQIPSPEKPSLRFILARHEPSERHRLFIRLSHALYDGVCFPAILNALKASYEGEPIATTPSYATYIHGLFSKANPDQHTYWRSLLEGSAPTNLIPRECRSMRTNPTQALRKIVATPSLATVNITTATVIKAAWSVVLAKNTGTRDVVFGHLISGRNSCHVPGIEAIVGPCLNVVPVRVQYQDSWMVLDLLQHIQHQQVDNIPHESLGFREIIRNCTNWDDDGANGFSTVVQHQSMAQTGSLDIGDNTYEVGVIASQEDTADFSVVTTPQDSSNTEVCFLYR |
Domain
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, methyltransferase domains (responsible for amino acid methylation) are present within the NRP synthetase. NRPS4 has the following architecture: A-T-E-C-A-T-C-A-T-E-C-A-T-C-A-T-E-C-T-Ct.
Sequence similarities
Belongs to the NRP synthetase family.
Keywords
- Domain