I1MFL2 · I1MFL2_SOYBN

Function

function

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by fructose 2,6-bisphosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site100diphosphate (UniProtKB | ChEBI)
Binding site194Mg2+ (UniProtKB | ChEBI); catalytic
Site195Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site221Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site222-224substrate
Active site224Proton acceptor
Binding site261-262substrate; ligand shared between dimeric partners
Binding site269-271substrate
Binding site330substrate
Binding site435-438substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processphotosynthesis
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
  • EC number
  • Short names
    PFP
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-PFK
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      100804429
    • Synonyms
      PFP-BETA
    • ORF names
      GLYMA_15G112300

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Williams 82
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja

Accessions

  • Primary accession
    I1MFL2

Proteomes

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Tetramer of two alpha (regulatory) and two beta (catalytic) chains.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain92-457Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    562
  • Mass (Da)
    61,264
  • Last updated
    2012-06-13 v1
  • Checksum
    92D1AD6E6006A8FB
MAPTTTVPIGTLPSGSVTGRLASVYSEVQKTRVDHALPLPSVLKHPFTILDGPPSSAAGNPDEIAKLFPHLFGQPSAALAPSGAVQTQKKLKIGVVLSGGQAPGGHNVISGIFDYLQERAPGSTLYGFKGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTSVIGCPKTIDGDLKCKEVPTSFGFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYIVDVVCKRAEINYNYGVILIPEGLIDFIPEVQHLIAELNEILAHDTVDEGGLWKKKLTDQSLKLFDFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQEGKYNGEFKGQSHFFGYEGRCGLPTNFDATYCYALGYGAGALLHSGKTGLISSVANLCAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASLRDEWALKNCYISPGPIQFSGPGSEVINHTLLLELEALA

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0R0FZ92A0A0R0FZ92_SOYBN100804429530

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000848
EMBL· GenBank· DDBJ
KRH11497.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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