I1IUJ6 · C90A1_BRADI
- Protein3beta,22alpha-dihydroxysteroid 3-dehydrogenase
- GeneCYP90A1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids498 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in reduction steps of the biosynthesis of plant campesterol-derivative steroids, ending to castasterone (CS) but missing brassinolide (BL) (PubMed:32146811).
Catalyzes the conversion of (22S)-22-hydroxycampesterol (22-hydroxyCR, 22-OHCR) to (22S,24R)-22-hydroxyergost-4-en-3-one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-one) and of 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:32146811).
Catalyzes the conversion of (22S)-22-hydroxycampesterol (22-hydroxyCR, 22-OHCR) to (22S,24R)-22-hydroxyergost-4-en-3-one (22-hydroxy-campesta-4-en-3-one, 22-OH-4-en-3-one) and of 6-deoxoteasterone (6-deoxoTE) to 3-dehydro-6-deoxoteasterone (6-deoxo3DT, 6-deoxo3DHT) (PubMed:32146811).
Catalytic activity
- (22S)-22-hydroxycampesterol + O2 + reduced [NADPH--hemoprotein reductase] = (22S)-22-hydroxycampest-4-en-3-one + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Plant hormone biosynthesis; brassinosteroid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | anther wall tapetum cell differentiation | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | brassinosteroid homeostasis | |
Biological Process | pollen exine formation | |
Biological Process | positive regulation of flower development | |
Biological Process | response to UV-B | |
Biological Process | sterol metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3beta,22alpha-dihydroxysteroid 3-dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Stipodae > Brachypodieae > Brachypodium
Accessions
- Primary accessionI1IUJ6
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 5-25 | Helical | ||||
Sequence: GALQLLSAAAAAVAVVVVVAL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455306 | 1-498 | 3beta,22alpha-dihydroxysteroid 3-dehydrogenase | |||
Sequence: MENAGALQLLSAAAAAVAVVVVVALRWLLARRAAGGWKQRPRLPPGSTGLPLIGETLRLISAYKTPDPEPFIDERVARHGGVFTTHVFGERTVFSADPAFNRLLLAAEGRAVDCSYPSSITTLLGARSLLLTRGAAHKRLHSLTLTRLGRPASPPLLAHIDRLVLDTMRHWEPAATVRLLDEAKKITFNLTVKQLVSIDPGPWTESLRREYVKLIDGFFSIPFPFASLLPFTTYGQALKSRKKVAGALREVIRKRMEERGEESGATEEAESKREKKDMVEELLQAEGGSFSEEEMVDFCLSLLVAGYETTSVLMTVAVKFLTETPAALAQLKEEHENMTNMKGENQPLEWSDYKSMTFTQCVINETLRVANIISGVFRRANTDIHFKGYTIPKGCKIFASFRAVHLNNDHYENARTFDPWRWQSNHKLQNEVGANLFTPFGGGPRLCPGYELARVVISVFLHHLVMRFSWEAAEEDRLVFFPTTRTLKGYPINLRQRS |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 257-277 | Disordered | ||||
Sequence: EERGEESGATEEAESKREKKD |
Sequence similarities
Belongs to the cytochrome P450 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length498
- Mass (Da)55,778
- Last updated2012-06-13 v1
- ChecksumEE47942BE1266032
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000883 EMBL· GenBank· DDBJ | KQJ92356.1 EMBL· GenBank· DDBJ | Genomic DNA |