I0GWU2 · I0GWU2_ACTM4

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site139O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • Ordered locus names
      AMIS_90

Organism names

Accessions

  • Primary accession
    I0GWU2

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region1-20Disordered
Domain28-486DNA topoisomerase type IIA
Motif547-553GyrA-box

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    837
  • Mass (Da)
    92,894
  • Last updated
    2012-06-13 v1
  • Checksum
    6DDD8DCC52790E5A
MTDTPEPPDGDETQDDAVGGVAQRVEPVGLEVEMQRSYLDYAMSVIVGRALPDVRDGLKPVHRKILYAMYDSGFRPDRGYVKCARVVGDVMGNYHPHGDSSIYDALVRMGQPWSLRYRLIDGNGNFGSPGNDPAAAMRYTEAKLSPLAMEMLRDIDEDTVDMQDNYDGRTKEPTILPARFPNLLVNGSEGIAVGMATKIPPHNLREVAAAVQWCLDNPEADEVTTLEALLEFVKGPDFPTYGLIVGQQAIQDAYRTGRGSIRMRAVVEVEEDARGRPCLVVTELPYQVNPDNLAERIAELVKEGKLTGIADIRDESSGRTGMRLILVLKRDAVAKVVLNNLYKHTQLQETFGANMLALVDGVPRTLNLAQFIRYYVEHQIEVIRRRTAYRLRKAEERAHILRGLAKALDMLDEVIALIRRSPTVEDSRQGLMQLLDVDEVQATAILDMQLRRLAALERQRIVDELAKIEIEIADLKDILAKPERQRAIVSDELGEIVQKYGDDRRTQIIPFDGEVSMEDLIAREDVVVTITRTGYAKRTKTDMYRSQKRGGKGVSGATLRQDDIVSHFFVISTHDWILFFTNKGRVYRAKAYELPEASRVAKGQHVANLLAFQPDEQIAQIIQIPNYGVAPYLVLATKNGLVKKTRLEEFDSNRSGGIIAINLREDDELVGAALAASEDDLLLVSKKAQAIRFNATDEALRPMGRATSGVIGMRFGDNDELLAMEVVREGMDVLVATNGGYAKRTPIEEYPVQGRGGKGVLTAKITERRGGLVGALVISPEDELFAITSNGGVIRTPVKPVRRTRDRNTMGVKLMDLPEGVTIVALARNADEPDEQD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012319
EMBL· GenBank· DDBJ
BAL85229.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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