I0B9T9 · I0B9T9_9BACL

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

1835100200300400500600700800
TypeIDPosition(s)Description
Active site123O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • ORF names
      B2K_00035

Organism names

Accessions

  • Primary accession
    I0B9T9

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain, coiled coil, motif, region, compositional bias.

TypeIDPosition(s)Description
Domain12-464DNA topoisomerase type IIA
Coiled coil436-477
Motif525-531GyrA-box
Region800-835Disordered
Compositional bias811-835Acidic residues

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    835
  • Mass (Da)
    93,876
  • Last updated
    2012-06-13 v1
  • Checksum
    260500EA93C8175B
MSEEPRSQVKEIDIGSEMRTSFMDYAMSIIVSRALPDVRDGLKPVHRRILFAMSELGMSPDKPYKKSARIVGEVIGKYHPHGDSAVYETMVRMAQDFSLRYMLVDGHGNFGSIDGDMAAAMRYTEARLSKIAMELLRDINKETIDFVPNYDGEEQEPAVLPSRFPNLLVNGLSGIAVGMATNIPPHNLTEVINGVQQLIQNPEVTPLELMQSIKGPDFPTGGYIMGREGIKQAYATGRGSVIMRAKTLIEENGNKARIIVYELPYQVNKARLIEKIAELVREKKIDGITDLRDESDRNGMRIVIELRRDVNPNVVLNNLYKQTAMQSNFGIIMLALVNGEPKVLNLRDVLFHYLEHQKVVIRRRTEFELRKAEARAHILEGLRIALDHLDEVIALIRASRTTEEAREGLMTRFGLSFDQAQAILDMRLQRLTGLERQKIEEEYAELLKKIAEYKAILADEQLVLAIISEELNEIKEKFGDERRSEITIGEESIEDEDLIPQSDVVITITHTGYIKRLPVTTYRSQRRGGKGIMGMDTKDKDFVEHLFVTNTHHYLLFFTNKGKVYRLKAYEIPDLSRTARGTPIINLIQIEQGETINAVIPVESFDSDKYLFFASKKGLVKKTSLDDYSNIRKGGLIAITLREDDDLIGVRLTDGNQSIIMGTAQGMSIHFPENEVRPMGRSATGVKGIQLDEEDHVIDMDVVHEDNSVLIVTSKGFGKRTPVSEYRLQSRGGKGIKTLNVTSKNGPVVGLKVVQEEEDLMIITAFGTVIRTSMSGISLMGRNTQGVRLINIREEDEVSTLARVEKSDDPADDAEGEESEEIQETSEGFDETSEE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias811-835Acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003422
EMBL· GenBank· DDBJ
AFH59136.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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