H9EVY5 · H9EVY5_MACMU
- ProteinPolyprenol reductase
- GeneSRD5A3
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids318 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).
Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism.
Catalytic activity
- 17beta-hydroxy-5alpha-androstan-3-one + NADP+ = H+ + NADPH + testosteroneThis reaction proceeds in the backward direction.
- androst-4-ene-3,17-dione + H+ + NADPH = 5alpha-androstan-3,17-dione + NADP+This reaction proceeds in the backward direction.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) activity | |
Molecular Function | polyprenol reductase activity | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process | |
Biological Process | polyprenol catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyprenol reductase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca
Accessions
- Primary accessionH9EVY5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 20-46 | Helical | ||||
Sequence: LTLTAAFLLTLLLQLLPPGLLPGCAIF | ||||||
Transmembrane | 79-100 | Helical | ||||
Sequence: FYIISVLWNGFLLWCLSQSLFL | ||||||
Transmembrane | 120-139 | Helical | ||||
Sequence: FQGGELALSAFLVLVFLWLH | ||||||
Transmembrane | 160-181 | Helical | ||||
Sequence: VVQYCFGLVYYVLVGLTVLSQV | ||||||
Transmembrane | 201-218 | Helical | ||||
Sequence: WFHILGMMMFIWSSAHQY | ||||||
Transmembrane | 278-299 | Helical | ||||
Sequence: WLVVTNVFFSQALSAFLSHQFY |
Keywords
- Cellular component
PTM/Processing
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 199-318 | 3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal | ||||
Sequence: ARWFHILGMMMFIWSSAHQYKCHVILGNLRKNKAGVVIHCNHRIPFGDWFEYVSSPNYLAELMIYVSMAVTFGFHNFTWWLVVTNVFFSQALSAFLSHQFYKSKFVSYPKHRKAFLPFLF |
Sequence similarities
Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length318
- Mass (Da)36,472
- Last updated2016-11-30 v1
- ChecksumE8D61583387FEBBE
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A5F8ACS8 | A0A5F8ACS8_MACMU | SRD5A3 | 308 | ||
F6R6P8 | F6R6P8_MACMU | SRD5A3 | 306 | ||
A0A5F8A3U7 | A0A5F8A3U7_MACMU | SRD5A3 | 346 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JU322788 EMBL· GenBank· DDBJ | AFE66544.1 EMBL· GenBank· DDBJ | mRNA |