H8ZK39 · H8ZK39_LITPA
- ProteinV(D)J recombination-activating protein 1
- GeneRag-1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids212 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 divalent metal cation per subunit. Mg2+ or Mn2+.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | endonuclease activity | |
Molecular Function | histone binding | |
Molecular Function | protein homodimerization activity | |
Molecular Function | sequence-specific DNA binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | B cell differentiation | |
Biological Process | chromatin organization | |
Biological Process | T cell differentiation in thymus | |
Biological Process | V(D)J recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameV(D)J recombination-activating protein 1
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Neobatrachia > Ranoidea > Ranidae > Lithobates
Accessions
- Primary accessionH8ZK39
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence
- Sequence statusFragment
- Length212
- Mass (Da)23,316
- Last updated2012-05-16 v1
- Checksum2A6364D7D68553C6
Features
Showing features for non-terminal residue, sequence uncertainty.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: S | ||||||
Sequence uncertainty | 175 | I or L | ||||
Sequence: X | ||||||
Non-terminal residue | 212 | |||||
Sequence: S |