H8XZW3 · H8XZW3_9INFA

Function

function

Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated RIGI ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Also binds poly(A) and U6 snRNA. Inhibits the integrated stress response (ISR) in the infected cell by blocking dsRNA binding by EIF2AK2/PKR and further phosphorylation of EIF2S1/EIF-2ALPHA. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication.

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Cellular Componenthost cell nucleus
Molecular Functionprotein serine/threonine kinase inhibitor activity
Molecular FunctionRNA binding
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity
Biological Processsymbiont-mediated suppression of host mRNA processing
Biological Processsymbiont-mediated suppression of host PKR/eIFalpha signaling
Biological Processsymbiont-mediated suppression of host type I interferon-mediated signaling pathway
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Non-structural protein 1
  • Short names
    NS1

Gene names

    • Name
      NS1
    • Synonyms
      NS

Organism names

  • Taxonomic identifier
  • Strain
    • A/KOL/46/2009
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Insthoviricetes > Articulavirales > Orthomyxoviridae > Alphainfluenzavirus > Alphainfluenzavirus influenzae > Influenza A virus

Accessions

  • Primary accession
    H8XZW3

Subcellular Location

Keywords

Interaction

Subunit

Homodimer. Interacts with host TRIM25 (via coiled coil); this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated RIGI CARD ubiquitination. Interacts with human EIF2AK2/PKR, CPSF4, IVNS1ABP and PABPN1.

Family & Domains

Features

Showing features for compositional bias, region.

Type
IDPosition(s)Description
Compositional bias205-223Polar residues
Region205-230Disordered

Domain

The dsRNA-binding region is required for suppression of RNA silencing.

Sequence similarities

Belongs to the influenza A viruses NS1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    230
  • Mass (Da)
    25,772
  • Last updated
    2012-05-16 v1
  • Checksum
    0622DD94D3AB71DE
MDSHTVSSFQVDCFLWHVRKQAADQDLGDAPFLDRLRRDQKSLKGRGSTLGLNIETATCVGKQIVERILKEESDEALKMTMASALASRYLTDMTVEEMSRDWFMLMPKQKVAGPLCVRMDQAIMDKNIILKANFSVIFDRLENLTLLRAFTEEGAIVGEISPLPSFPGHTNEDVKNAIGVLIGGLEWNDNTVRVSETLQRFAWRSSNETGGPPFTTTQKRKMAGTTRSEV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias205-223Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HQ853516
EMBL· GenBank· DDBJ
ADV39813.1
EMBL· GenBank· DDBJ
Viral cRNA

Similar Proteins

Disclaimer

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