H8GAG2 · H8GAG2_9PSEU
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1127 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 117 | ATP (UniProtKB | ChEBI) | |||
Binding site | 200 | ATP (UniProtKB | ChEBI) | |||
Binding site | 238 | ATP (UniProtKB | ChEBI) | |||
Active site | 295 | ||||
Binding site | 536 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 608 | substrate | |||
Binding site | 706 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 735 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 737 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 870 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Saccharomonospora
Accessions
- Primary accessionH8GAG2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 706 | N6-carboxylysine | |||
Modified residue | 1093 | N6-biotinyllysine | |||
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-453 | Biotin carboxylation | |||
Domain | 121-320 | ATP-grasp | |||
Region | 490-509 | Disordered | |||
Domain | 527-796 | Pyruvate carboxyltransferase | |||
Domain | 1053-1127 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,127
- Mass (Da)121,215
- Last updated2012-05-16 v1
- Checksum3BAFAAFCC2C41DE0
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001466 EMBL· GenBank· DDBJ | EHY90627.1 EMBL· GenBank· DDBJ | Genomic DNA |