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H8F3Q9 · KATG_MYCTE

Function

function

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity, oxidizing various electron donors including NADP(H) (By similarity).
Protects M.tuberculosis against toxic reactive oxygen species (ROS) including hydrogen peroxide as well as organic peroxides and thus contributes to its survival within host macrophages by countering the phagocyte oxidative burst (PubMed:15165233).
Also displays efficient peroxynitritase activity, which may help the bacterium to persist in macrophages (By similarity).
Catalyzes the oxidative activation of the antitubercular pro-drug isoniazid (INH) to generate an isonicotinoyl radical that then reacts nonenzymatically with NAD to form an isonicotinoyl-NAD adduct which inhibits InhA.

Catalytic activity

Cofactor

heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.

Features

Showing features for site, active site, binding site.

Type
IDPosition(s)Description
Site104Transition state stabilizer
Active site108Proton acceptor
Binding site270Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); axial binding residue
Active site321Tryptophan radical intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functioncatalase activity
Molecular Functionheme binding
Molecular Functionmetal ion binding
Biological Processcellular response to hydrogen peroxide
Biological Processhydrogen peroxide catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Catalase-peroxidase
  • EC number
  • Short names
    CP
  • Alternative names
    • Peroxidase/catalase

Gene names

    • Name
      katG
    • Ordered locus names
      ERDMAN_2101

Organism names

Accessions

  • Primary accession
    H8F3Q9

Proteomes

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene are devoid of catalase activity, supersensitive to H2O2 exposure and highly resistant to the antitubercular drug isoniazid (INH) in vitro. This mutant strain is markedly attenuated for virulence in mice and displays impaired growth in infected macrophages, but its growth and survival is indistinguishable from wild-type in macrophages lacking the ROS-generating NADPH oxidase (Phox).

PTM/Processing

Features

Showing features for chain, cross-link.

Type
IDPosition(s)Description
ChainPRO_00004226881-740Catalase-peroxidase
Cross-link107↔229Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255)
Cross-link229↔255Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107)

Post-translational modification

Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.

Keywords

Expression

Induction

By the metal chelator phenanthroline via Rip1, RskA/SigK and RslA/SigL.

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Sequence similarities

Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    740
  • Mass (Da)
    80,605
  • Last updated
    2012-05-16 v1
  • MD5 Checksum
    D08E940EC2068562479EBDBDAD3F75BC
MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVATIDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNASLDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERYSGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGPADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGAWQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDMGPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIRASGLTVSQLVSTAWAAASSFRGSDKRGGANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAGHNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGGLRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGSNSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012340
EMBL· GenBank· DDBJ
BAL65894.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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