H8EXN1 · SIGL_MYCTE

Function

function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (By similarity).

Miscellaneous

Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning anti-sigma factor then within the membrane itself (site-2 protease, S2P, Rip1), while cytoplasmic proteases finish degrading the regulatory protein, liberating SigL (Probable).

Features

Showing features for dna binding.

117720406080100120140160
TypeIDPosition(s)Description
DNA binding141-160H-T-H motif

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionsigma factor activity
Biological ProcessDNA-templated transcription initiation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ECF RNA polymerase sigma factor SigL
  • Short names
    ECF sigma factor SigL
  • Alternative names
    • Alternative RNA polymerase sigma factor SigL
    • RNA polymerase sigma-L factor (Sigma-L factor)

Gene names

    • Name
      sigL
    • Ordered locus names
      ERDMAN_0808

Organism names

Accessions

  • Primary accession
    H8EXN1

Proteomes

Phenotypes & Variants

Disruption phenotype

Loss of phenanthroline induction of katG.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004226931-177ECF RNA polymerase sigma factor SigL

Interaction

Subunit

Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription. Interacts (via sigma-70 factor domain 4) with anti-sigma-L factor RslA (By similarity).

Structure

Family & Domains

Features

Showing features for region, motif.

Type
IDPosition(s)Description
Region18-85Sigma-70 factor domain-2
Motif42-45Interaction with polymerase core subunit RpoC
Region119-167Sigma-70 factor domain-4

Domain

The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).

Sequence similarities

Belongs to the sigma-70 factor family. ECF subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    177
  • Mass (Da)
    19,538
  • Last updated
    2012-05-16 v1
  • Checksum
    8E13D528AEDD9E16
MARVSGAAAAEAALMRALYDEHAAVLWRYALRLTGDAAQAEDVVQETLLRAWQHPEVIGDTARPARAWLFTVARNMIIDERRSARFRNVVGSTDQSGTPEQSTPDEVNAALDRLLIADALAQLSAEHRAVIQRSYYRGWSTAQIATDLGIAEGTVKSRLHYAVRALRLTLQELGVTR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012340
EMBL· GenBank· DDBJ
BAL64620.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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