H8EXN1 · SIGL_MYCTE
- ProteinECF RNA polymerase sigma factor SigL
- GenesigL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids177 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (By similarity).
Miscellaneous
Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning anti-sigma factor then within the membrane itself (site-2 protease, S2P, Rip1), while cytoplasmic proteases finish degrading the regulatory protein, liberating SigL (Probable).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
DNA binding | 141-160 | H-T-H motif | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | sigma factor activity | |
Biological Process | DNA-templated transcription initiation |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameECF RNA polymerase sigma factor SigL
- Short namesECF sigma factor SigL
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionH8EXN1
Proteomes
Phenotypes & Variants
Disruption phenotype
Loss of phenanthroline induction of katG.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000422693 | 1-177 | ECF RNA polymerase sigma factor SigL | ||
Interaction
Subunit
Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription. Interacts (via sigma-70 factor domain 4) with anti-sigma-L factor RslA (By similarity).
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 18-85 | Sigma-70 factor domain-2 | |||
Motif | 42-45 | Interaction with polymerase core subunit RpoC | |||
Region | 119-167 | Sigma-70 factor domain-4 | |||
Domain
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).
Sequence similarities
Belongs to the sigma-70 factor family. ECF subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length177
- Mass (Da)19,538
- Last updated2012-05-16 v1
- Checksum8E13D528AEDD9E16
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012340 EMBL· GenBank· DDBJ | BAL64620.1 EMBL· GenBank· DDBJ | Genomic DNA |