H8EVV4 · ACEA1_MYCTE

Function

function

Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It also catalyzes the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

Miscellaneous

Cell growing on 0.2% glucose show barely detectable isocitrate and methylisocitrate lyase activities. On 0.1% and 0.2% propionate, the lyase activities increase more than 10 and 100-fold, respectively.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site91-93substrate
Binding site153Mg2+ (UniProtKB | ChEBI)
Active site191Proton acceptor
Binding site192-193substrate
Binding site228substrate
Binding site313-317substrate
Binding site347substrate

GO annotations

AspectTerm
Molecular Functionisocitrate lyase activity
Molecular Functionmetal ion binding
Molecular Functionmethylisocitrate lyase activity
Biological Processglyoxylate cycle
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isocitrate lyase 1
  • EC number
  • Short names
    ICL1
  • Alternative names
    • Isocitrase
    • Isocitratase
    • Methylisocitrate lyase
      (MICA
      ) (EC:4.1.3.30
      ) . EC:4.1.3.30 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      icl1
    • ORF names
      Q643_00485
    • Ordered locus names
      ERDMAN_0512

Organism names

Accessions

  • Primary accession
    H8EVV4

Proteomes

Phenotypes & Variants

Disruption phenotype

Deletion of icl1 in cells growing on 0.1% propionate shows 10 and 8-fold decrease of isocitrate and methylisocitrate lyase activity, respectively.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004325641-428Isocitrate lyase 1

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    428
  • Mass (Da)
    47,087
  • Last updated
    2012-05-16 v1
  • Checksum
    E5223F38CB5D9E8B
MSVVGTPKSAEQIQQEWDTNPRWKDVTRTYSAEDVVALQGSVVEEHTLARRGAEVLWEQLHDLEWVNALGALTGNMAVQQVRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPQVVRRINNALQRADQIAKIEGDTSVENWLAPIVADGEAGFGGALNVYELQKALIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVADVPTVVIARTDAEAATLITSDVDERDQPFITGERTREGFYRTKNGIEPCIARAKAYAPFADLIWMETGTPDLEAARQFSEAVKAEYPDQMLAYNCSPSFNWKKHLDDATIAKFQKELAAMGFKFQFITLAGFHALNYSMFDLAYGYAQNQMSAYVELQEREFAAEERGYTATKHQREVGAGYFDRIATTVDPNSSTTALTGSTEEGQFH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012340
EMBL· GenBank· DDBJ
BAL64328.1
EMBL· GenBank· DDBJ
Genomic DNA
JLBG01000002
EMBL· GenBank· DDBJ
KBK18542.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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