H8EVV4 · ACEA1_MYCTE
- ProteinIsocitrate lyase 1
- Geneicl1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids428 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It also catalyzes the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).
Miscellaneous
Cell growing on 0.2% glucose show barely detectable isocitrate and methylisocitrate lyase activities. On 0.1% and 0.2% propionate, the lyase activities increase more than 10 and 100-fold, respectively.
Catalytic activity
- D-threo-isocitrate = glyoxylate + succinate
Cofactor
Pathway
Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 91-93 | substrate | ||||
Sequence: SGW | ||||||
Binding site | 153 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 191 | Proton acceptor | ||||
Sequence: C | ||||||
Binding site | 192-193 | substrate | ||||
Sequence: GH | ||||||
Binding site | 228 | substrate | ||||
Sequence: R | ||||||
Binding site | 313-317 | substrate | ||||
Sequence: NCSPS | ||||||
Binding site | 347 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | isocitrate lyase activity | |
Molecular Function | metal ion binding | |
Molecular Function | methylisocitrate lyase activity | |
Biological Process | glyoxylate cycle | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameIsocitrate lyase 1
- EC number
- Short namesICL1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionH8EVV4
Proteomes
Phenotypes & Variants
Disruption phenotype
Deletion of icl1 in cells growing on 0.1% propionate shows 10 and 8-fold decrease of isocitrate and methylisocitrate lyase activity, respectively.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000432564 | 1-428 | Isocitrate lyase 1 | |||
Sequence: MSVVGTPKSAEQIQQEWDTNPRWKDVTRTYSAEDVVALQGSVVEEHTLARRGAEVLWEQLHDLEWVNALGALTGNMAVQQVRAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPQVVRRINNALQRADQIAKIEGDTSVENWLAPIVADGEAGFGGALNVYELQKALIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADVADVPTVVIARTDAEAATLITSDVDERDQPFITGERTREGFYRTKNGIEPCIARAKAYAPFADLIWMETGTPDLEAARQFSEAVKAEYPDQMLAYNCSPSFNWKKHLDDATIAKFQKELAAMGFKFQFITLAGFHALNYSMFDLAYGYAQNQMSAYVELQEREFAAEERGYTATKHQREVGAGYFDRIATTVDPNSSTTALTGSTEEGQFH |
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length428
- Mass (Da)47,087
- Last updated2012-05-16 v1
- ChecksumE5223F38CB5D9E8B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012340 EMBL· GenBank· DDBJ | BAL64328.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JLBG01000002 EMBL· GenBank· DDBJ | KBK18542.1 EMBL· GenBank· DDBJ | Genomic DNA |