H3N136 · H3N136_9ENTR

  • Protein
    Dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site109Proton acceptor
Binding site129[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site133[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site136[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site183-184S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site215S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site237-239S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site316S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site359S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      CWM85_12065
      , CWN49_08495
      , DN619_06645
      , L373_01988
      , N5C89_19370
      , N7692_17215

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • MGH 27
    • A10
    • A2
    • GEO_33_Up_A
    • GD03918
    • GD03999
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella

Accessions

  • Primary accession
    H3N136
  • Secondary accessions
    • A0A0E0X2L9

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond122↔359(transient)

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain115-354Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    388
  • Mass (Da)
    43,272
  • Last updated
    2012-04-18 v1
  • Checksum
    62EC43882320AAB6
MSEQIVTPDSAALTVPNKDAKINLLDLNRQQLREFFKNMGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAVKTTGVRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDTIRDEIVPINKKYNIETFLNSVRGYISKSNANQGRVTIEYVMLDHVNDGTEHAHELAALLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDVKAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JCNZ01000008
EMBL· GenBank· DDBJ
EWF89493.1
EMBL· GenBank· DDBJ
Genomic DNA
JAOEEU010000019
EMBL· GenBank· DDBJ
MDH0489346.1
EMBL· GenBank· DDBJ
Genomic DNA
JAOCBF010000027
EMBL· GenBank· DDBJ
MDH0965000.1
EMBL· GenBank· DDBJ
Genomic DNA
PIET01000290
EMBL· GenBank· DDBJ
PLM64231.1
EMBL· GenBank· DDBJ
Genomic DNA
PIDR01000181
EMBL· GenBank· DDBJ
PLO72126.1
EMBL· GenBank· DDBJ
Genomic DNA
QKPB01000009
EMBL· GenBank· DDBJ
RWT46913.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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