H2N0D4 · AMY_ORYLA
- ProteinAlpha-amylase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids512 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of alpha-1,4 glycosidic linkages in starch, glycogen and similar oligosaccharides.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Ca2+ ion per subunit.
Note: Binds 1 Cl- ion per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1.18 mg/ml | potato amylopectin |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
2560 μmol/min/mg |
pH Dependence
Optimum pH is 7.1.
Temperature Dependence
Optimum temperature is 50 degrees Celsius.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 115 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 173 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 182 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 210 | chloride (UniProtKB | ChEBI) | |||
Active site | 212 | Nucleophile | |||
Binding site | 216 | Ca2+ (UniProtKB | ChEBI) | |||
Active site | 248 | Proton donor | |||
Site | 315 | Transition state stabilizer | |||
Binding site | 352 | chloride (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Molecular Function | alpha-amylase activity | |
Molecular Function | metal ion binding | |
Biological Process | carbohydrate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlpha-amylase
- EC number
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Neoteleostei > Acanthomorphata > Ovalentaria > Atherinomorphae > Beloniformes > Adrianichthyidae > Oryziinae > Oryzias
Accessions
- Primary accessionH2N0D4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-15 | ||||
Chain | PRO_5012994493 | 16-512 | Alpha-amylase | ||
Disulfide bond | 43↔101 | ||||
Disulfide bond | 85↔130 | ||||
Disulfide bond | 156↔175 | ||||
Disulfide bond | 394↔400 | ||||
Disulfide bond | 466↔478 | ||||
Glycosylation | 496 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length512
- Mass (Da)57,167
- Last updated2012-03-21 v1
- MD5 Checksum55E37258B3E85C1A22F0488993FCDF70
Keywords
- Technical term