H2IU49 · H2IU49_RAHAC

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s2U34, the first step of tRNA-mnm5s2U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.

Features

Showing features for binding site, active site, site.

136950100150200250300350
TypeIDPosition(s)Description
Binding site12-19ATP (UniProtKB | ChEBI)
Binding site38ATP (UniProtKB | ChEBI)
Active site103Nucleophile
Binding site128ATP (UniProtKB | ChEBI)
Site129Interaction with tRNA
Active site200Cysteine persulfide intermediate
Site345Interaction with tRNA

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmethyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological Processmethylation
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • Ordered locus names
      Rahaq2_2947

Organism names

Accessions

  • Primary accession
    H2IU49

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond103↔200Alternate

Keywords

Interaction

Subunit

Interacts with TusE.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region98-100Interaction with target base in tRNA
Region150-152Interaction with tRNA
Domain208-276tRNA-specific 2-thiouridylase MnmA-like central
Domain287-361tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region312-313Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    41,126
  • Last updated
    2012-03-21 v1
  • Checksum
    F0800920F654ECB3
MSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYQVEGLFMKNWEEDDDEEYCTAATDLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADYIATGHYVRRQDIDGVSHLLRGVDGNKDQSYFLYTLGHEQVAQSLFPVGELEKPEVRRIAEELDLITAKKKDSTGICFIGERKFRDFLGRYLPAQPGVIKSVDGQVMGEHQGLMYHTLGQRKGLGIGGQKDGSEEPWYTVEKDVENNILYVAQGHEHPRLMSVGLIAQQLHWVDRKTLTAPLTCTVKTRYRQEDIPCVVTPLGDDRIEVHFDAPVAAVTPGQSAVFYLGDVCLGGGVIEQRLPLQA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003244
EMBL· GenBank· DDBJ
AEX52773.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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