H2IU49 · H2IU49_RAHAC
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids369 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln), leading to the formation of s2U34, the first step of tRNA-mnm5s2U34 synthesis. Sulfur is provided by IscS, via a sulfur-relay system. Binds ATP and its substrate tRNAs.
Catalytic activity
- AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA = 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 + RHEA-COMP:11727 CHEBI:65315 Position: 34= RHEA-COMP:11728 CHEBI:87170 Position: 34+ CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-19 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GMSGGVDS | ||||||
Binding site | 38 | ATP (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Active site | 103 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 128 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 129 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 200 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 345 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | methyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | methylation | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Rahnella
Accessions
- Primary accessionH2IU49
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 103↔200 | Alternate | ||||
Sequence: CNKEIKFKAFLEFAAEDLGADYIATGHYVRRQDIDGVSHLLRGVDGNKDQSYFLYTLGHEQVAQSLFPVGELEKPEVRRIAEELDLITAKKKDSTGIC |
Keywords
- PTM
Interaction
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 98-100 | Interaction with target base in tRNA | ||||
Sequence: NPD | ||||||
Region | 150-152 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 208-276 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: RDFLGRYLPAQPGVIKSVDGQVMGEHQGLMYHTLGQRKGLGIGGQKDGSEEPWYTVEKDVENNILYVAQ | ||||||
Domain | 287-361 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: GLIAQQLHWVDRKTLTAPLTCTVKTRYRQEDIPCVVTPLGDDRIEVHFDAPVAAVTPGQSAVFYLGDVCLGGGVI | ||||||
Region | 312-313 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)41,126
- Last updated2012-03-21 v1
- ChecksumF0800920F654ECB3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003244 EMBL· GenBank· DDBJ | AEX52773.1 EMBL· GenBank· DDBJ | Genomic DNA |