H1VQW0 · DPCHF_COLHI

Function

function

FAD-dependent oxidoreductase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Biotechnology

Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent oxidoreductase dpchF
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein F

Gene names

    • Name
      dpchF
    • ORF names
      CH063_12564, CH63R_05482

Organism names

Accessions

  • Primary accession
    H1VQW0

Proteomes

Organism-specific databases

Phenotypes & Variants

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-21
ChainPRO_501049773522-473FAD-dependent oxidoreductase dpchF
Glycosylation98N-linked (GlcNAc...) asparagine
Glycosylation128N-linked (GlcNAc...) asparagine
Glycosylation181N-linked (GlcNAc...) asparagine
Glycosylation262N-linked (GlcNAc...) asparagine
Glycosylation330N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    473
  • Mass (Da)
    50,041
  • Last updated
    2012-03-21 v1
  • Checksum
    F55B664135600AC4
MKLSFIASPVWALALAQFAAATQVKIDVDVAIFGGGSAGIHAAIQLRDAGATVAVIEKKSQIGGHAETYTDPQGKSTNVGVVVFDNIEVASNYFARLNVSIVRGSPLGTAGPTYTYDFTSGAQIPAVNTSAEAQQQLTAALQSYSTNVLSKYPWIDEGFLVPDPVPEELTIPFGELAQKYNFTALMPTIAMYNYFTGDLSTIPSLYGIKGLGQGALKNLFGSFILPASGKTRDLYDAAAIELGNSVLLNADVVKVQRDVRINSTTTGVTVLIQQPGQPPKLIRARKLLVAAPPTLENVGAFDLTAEERGLISKFSSLGCWASVANVPGLNVTLKNYGVHMPYNQPSIPGPYGFVAYGSPNNFLVTVGLPDAANTAAKGEAVVRQSLATLSAVGAVPADALEKLTFPFSAVHSPYSLRVSAEEIKAGFYSKFLALEGARNTYWTGAVWAGHNSALIWNFNMGTVLPGLKKDLGL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LTAN01000004
EMBL· GenBank· DDBJ
OBR09790.1
EMBL· GenBank· DDBJ
Genomic DNA
CACQ02005519
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp