H1VN83 · DPCHG_COLHI
- ProteinShort chain dehydrogenase/reductase dpchG
- GenedpchG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids268 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).
Biotechnology
Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 18 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 70 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 97 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 131 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 165 | Proton acceptor | ||||
Sequence: Y | ||||||
Binding site | 165 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Active site | 169 | Lowers pKa of active site Tyr | ||||
Sequence: K | ||||||
Binding site | 169 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameShort chain dehydrogenase/reductase dpchG
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Glomerellales > Glomerellaceae > Colletotrichum > Colletotrichum destructivum species complex
Accessions
- Primary accessionH1VN83
Proteomes
Organism-specific databases
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451549 | 1-268 | Short chain dehydrogenase/reductase dpchG | |||
Sequence: MTSSEKNSTGLAGKTCLVTGGAGGLGKAVTAAFLDAGANVVICDINAKRIESTLAELRTRGGNLTAVTADITDHDQVLSLFDDIAGRFGTLDVLVNNAAVMDRFDPVGDIELDLWDNVLAVNLTAPLQLSKFAVRSMLSKPEPAGCIINIASGAATAGWLAGTAYTASKHGLVGLTKSTAAFYGPKGIRCNALIMGVMVGTHMHEAFLDGCHKEGRQKVEEIFSGHRPQTCKVDDVAGICLSLASGPGWGTVNGALIAVDNGWTSVVG |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length268
- Mass (Da)27,687
- Last updated2012-03-21 v1
- Checksum16EF74BB6E672EA1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM004458 EMBL· GenBank· DDBJ | OBR09785.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACQ02004858 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |