H1VN83 · DPCHG_COLHI

Function

function

Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of the diterpenoid pyrones higginsianins A and B (PubMed:32286350).
The first step of the pathway is the synthesis of the alpha-pyrone moiety by the polyketide synthase dpchA via condensation of one acetyl-CoA starter unit with 3 malonyl-CoA units and 2 methylations (Probable). The alpha-pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed by the GGPP synthase dpchD through the action of the prenyltransferase dpchC to yield a linear alpha-pyrone diterpenoid (Probable). Subsequent steps in the diterpenoid pyrone biosynthetic pathway involve the decalin core formation, which is initiated by the epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase dpchE, and is followed by a cyclization cascade catalyzed by the terpene cyclase dpchB (Probable). The short chain dehydrogenase/reductase dpchG then oxidizes the 8S hydroxy group to a ketone and the short chain dehydrogenase/reductase dpchH reduces the ketone to the 8R hydroxy group to yield higginsianin B (PubMed:32286350).
Finally, the FAD-dependent oxidoreductase dpchF converts higginsianin B into higginsianin A (PubMed:32286350).

Biotechnology

Diterpenoid pyrones display various biological activities and higginsianin A shows anti-HIV activity.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site18NADP+ (UniProtKB | ChEBI)
Binding site70NADP+ (UniProtKB | ChEBI)
Binding site97NADP+ (UniProtKB | ChEBI)
Binding site131NADP+ (UniProtKB | ChEBI)
Active site165Proton acceptor
Binding site165NADP+ (UniProtKB | ChEBI)
Active site169Lowers pKa of active site Tyr
Binding site169NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Short chain dehydrogenase/reductase dpchG
  • EC number
  • Alternative names
    • Diterpenoid pyrone biosynthesis cluster protein G

Gene names

    • Name
      dpchG
    • ORF names
      CH063_11894, CH63R_05477

Organism names

Accessions

  • Primary accession
    H1VN83

Proteomes

Organism-specific databases

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004515491-268Short chain dehydrogenase/reductase dpchG

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    268
  • Mass (Da)
    27,687
  • Last updated
    2012-03-21 v1
  • Checksum
    16EF74BB6E672EA1
MTSSEKNSTGLAGKTCLVTGGAGGLGKAVTAAFLDAGANVVICDINAKRIESTLAELRTRGGNLTAVTADITDHDQVLSLFDDIAGRFGTLDVLVNNAAVMDRFDPVGDIELDLWDNVLAVNLTAPLQLSKFAVRSMLSKPEPAGCIINIASGAATAGWLAGTAYTASKHGLVGLTKSTAAFYGPKGIRCNALIMGVMVGTHMHEAFLDGCHKEGRQKVEEIFSGHRPQTCKVDDVAGICLSLASGPGWGTVNGALIAVDNGWTSVVG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM004458
EMBL· GenBank· DDBJ
OBR09785.1
EMBL· GenBank· DDBJ
Genomic DNA
CACQ02004858
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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