H1KHX8 · H1KHX8_METEX
- ProteinFormamidopyrimidine-DNA glycosylase
- GenemutM
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids299 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 2 | Schiff-base intermediate with DNA | ||||
Sequence: P | ||||||
Active site | 3 | Proton donor | ||||
Sequence: E | ||||||
Active site | 58 | Proton donor; for beta-elimination activity | ||||
Sequence: K | ||||||
Binding site | 106 | DNA (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 125 | DNA (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 168 | DNA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 285 | Proton donor; for delta-elimination activity | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | damaged DNA binding | |
Molecular Function | oxidized purine nucleobase lesion DNA N-glycosylase activity | |
Molecular Function | zinc ion binding | |
Biological Process | base-excision repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFormamidopyrimidine-DNA glycosylase
- EC number
- Short namesFapy-DNA glycosylase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylobacteriaceae > Methylorubrum
Accessions
- Primary accessionH1KHX8
Proteomes
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-128 | Formamidopyrimidine-DNA glycosylase catalytic | ||||
Sequence: PELPEVETVRRGLAPAMVGARVARVTLRRPNLRFPFPERFAERLEGTTVLELARRAKYLTAHLDSGESLILHLGMSGRFDVRLPDGSNLSPGDFYLEGALGTPKHDHVVMVFANGATVTYNDARRFG | ||||||
Domain | 259-295 | FPG-type | ||||
Sequence: RVYDRVGHACPTKGCTGRVGRIVQGGRSTFFCETCQV |
Sequence similarities
Belongs to the FPG family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length299
- Mass (Da)32,463
- Last updated2012-03-21 v1
- ChecksumF28AAECBA6F9C143
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGJK01000047 EMBL· GenBank· DDBJ | EHP92880.1 EMBL· GenBank· DDBJ | Genomic DNA |